摘要
目的 探讨端粒酶和原癌基因c_myc和p5 3在舌癌组织中的表达以及相互作用 ,分析它们与舌癌组织临床病理特征之间的可能关系。方法 采用原位杂交技术和端粒末端重复放大技术 (TRAP)分别检测c_myc和p5 3在舌癌组织中的表达以及舌癌组织中端粒酶的活性。结果 在低分化的舌癌组织中端粒酶活性明显升高(P <0 0 5 ) ,c_myc在低分化 1级舌癌组织中阳性表达显著升高 (P <0 0 5 ) ;p5 3在舌癌伴淋巴结转移患者中阳性表达明显降低 (P <0 0 5 )。结论 端粒酶在舌癌的发生发展过程中可能起重要作用 ,c_myc和p5 3在舌癌发生过程中对端粒酶的激活发挥重要影响。
Objective To study telomerase activity and expression of oncogenes c-myc and p53 in tongue cancer, analyse the interaction among them, and assess their possible correlations with tongue cancer clinicopathological features.Methods To detective the telomerase activity by TRAP and examine the positive expression of c-myc and p53 in tongue cancer by hybridization in situ.Results A high telomerase activity existed in lower differentiated tongue cancer (P<0.05); the positive expression of c-myc increased significantly in lower grade tongue cancer (P<0.05) and the positive expression of p53 decreased increasingly in tongue cancer accompanied with lymph node metastasis (P<0.05).Conclusion Telomerase may play a key role in the tumorigenesis of tongue cancer. Meantime, c-myc and p53 exerts important effect on telomerase activation during the course of tongue cancer generation and development.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2003年第4期274-276,共3页
West China Journal of Stomatology
基金
国家自然科学基金青年基金资助项目 (编号 3990 0 0 36)
