摘要
各种不同类型的植物蛋白酶抑制剂基因已被分离 ,它们的特异产物 (单基因或多基因组合 ) ,对昆虫体内各种生化和生理过程会产生不同程度的影响 ,在对昆虫和病原体防御体系中起重要作用。多种蛋白酶抑制剂重组 ,协同保护植物的方法 ,已成为害虫综合防治计划的一部分。尽管它们近期内尚不能代替化学杀虫剂 ,但可作为有效的替补。目前 ,大多数抑制剂的作用和机理正在详尽地研究中 ,该文综述了植物蛋白酶抑制剂的基因结构、调控与表达并讨论了培育转基因作物控制害虫的策略。
The diverse plant proteinase inhibitors (PIs) genes from different plant species have been isolated and their products with one or more genes are targeted at different biochemical and physiological process with the insect,and well played a potent defensive role against insects and pathogens.The use of recombinant PIs and synergistic activation to protect plants has been incorporated in integrated pest management program.Though they may not replace the use of chemical pesticides in the near future,but effectively complement it.Currently,the role and mechanism of action for most of these inhibitors are being studied in detail.This article describes the structure of PIs genes,their regulation and expression,and discusses the strategy to develop transgenic plants against phytophagous insects.
基金
国家 8 63计划 (10 1 0 1 0 1 0 1 3 )
国家自然科学基金 (3 9870 42 1)资助~~
关键词
植物蛋白酶抑制剂基因
调控与表达
转基因
表达
plant proteinase inhibitor gene
structure and regulation
transgene
expression
