摘要
利用来航鸡胚,于孵育第3d,从气室注入0.2ml/卵AlCl_3水溶液(1.708μmol/卵)。取给药组与对照组第4d和第6d神经管及第6d以后小脑,在光镜及电镜下观察;并用~3H-TdR或~3H-methionine标记。发现鸡胚神经管形成后,生发层细胞的~3H-TdR标记率以翼板区最高,其次是基板区;给药组标记率低于对照组;神经管生发层细胞有丝分裂相减少;套层细胞的尼氏体(光镜下)、粗面内质网和游离核蛋白体(电镜下)减少。
The white Lehorn chick embryos were used in this study, On the third day of incubation, AlCl_3 solution(0.2ml) (1.708 μmol/egg)was injected into the air sac. The neural tube of the chick embryos within the 4～6d & cerebellum of the chick embryos beyond 6d were taken from both experiment & control groups & obsreved under light & electron microscopes, and labelled with ~3H-TdR & ~3H-methionine. We found that after formation of the neural tube in normal chick embryos, the incorporation rates of ~3H-TdR with cells of the germinal layer were the highest in the alar plate, next in the basal plate, while the incorporation rates of AlCl_3-treated groups were lower than those in controls. The mitotic figures were reduced in the germinal layers of the neural tube (4-6th d) &the cerebellum primordium on the 6th d. The number of Nissl bodies (under light microscope) & the number of rough endoplasmic reticula) & free ribosorues (under electron microscope) were reduced in cells of the mantle layer of AlCl_3-treated groups.
出处
《安徽医科大学学报》
CAS
1989年第2期92-96,共5页
Acta Universitatis Medicinalis Anhui
关键词
鸡胚
铝
毒性
畸变
超微结构
chick embryo
aluminium/tox
Nissl bodies
Purkinje cells/ultrastruct
mitosis
