摘要
目的 观察人胚来源的神经干细胞在正常成人脑脊液中的分化情况并探讨其机制。方法 将原代培养的人胚来源的神经干细胞置入含有正常成人脑脊液培养基 (DMEM/F12 +B2 7+ 3 0 %脑脊液 )使其分化 ,在分化的不同时间对分化的细胞进行免疫细胞化学染色 ,计数产生的各类神经细胞的比例。结果 正常成人脑脊液能诱导人胚来源的神经干细胞分化成神经元、星型胶质细胞、少突胶质细胞 ,与其在胎牛血清中的分化相比星型胶质细胞比例增多 (P <0 0 5 ) ,而其他类型细胞比例无显著性差异。结论 正常成人脑脊液能诱导人胚来源的神经干细胞分化成神经元、星型胶质细胞、少突胶质细胞 ,神经干细胞在脑脊液中可以存活和分化 。
Objective: To observe the fetal brain derived neural stem cells' differentiation in the normal adult cerebrospinal fluid and investigate the potential mechanism. Methods: The primary culture of fetal brain derived neural stem cells were transferred into the medium DMEM/F12+B27+30%csf and DMEM/F12+B27+10%fbs. At different time points the differentiated cells are immuno-stained and the various types of cells are counted. Results: The neural stem cells can differentiate into neurons,astrocytos′and oligodendrocytos in normal csf. The proportion of neurons is significantly increase compared to their differentiation in serum-containing medium. Conclusions: In normal csf the neural stem cells can survive and differentiate into neurons,astrocytos′and oligodendrocytos, which provides support of the neurotransplantation using neural stem cells.
出处
《实用临床医药杂志》
CAS
2003年第3期202-204,211,共4页
Journal of Clinical Medicine in Practice
基金
国家自然科学基金资助项目 (3 0 2 713 2 5 )
江苏省自然科学基金资助项目 (BK2 0 0 1170 )
关键词
神经干细胞
分化
脑脊液
神经移植
neural stem cells
differentiation
cerebrospinal fluid
neurotransplantation
