摘要
目的检测p27蛋白在人髓母细胞瘤中的表达及定位情况,探讨p27与髓母细胞瘤预后的关系。方法应用免疫组化SP法和激光共聚焦显微术检测57例髓母细胞瘤标本中p27蛋白的表达及其定位情况,结合随访资料进行生存分析。结果在全部57例髓母细胞瘤标本中,p27阳性表达为32.41%～96.53%,平均为64.34%±18.10%,Kaplan-Meier生存曲线分析示p27高表达组≥80%较低表达组(<80%)有显著性差异(P<0.0001);以p27阳性表达率60%为截取值,两组间生存曲线有显著性差异(P<0.0001)。激光共聚焦显微术检测显示p27在肿瘤细胞质中有表达,生存曲线分析显示在全部病例及儿童病例组中有显著性差异(P<0.05),而在成人病例组中则无显著性差异P>0.05。Cox比例风险模型分析显示p27表达降低和p27在细胞质中呈阳性表达在髓母细胞瘤中的相对危险度分别为4.5(95%CI2.3～6.7)、3.4(95%CI1.6～4.1)。结论在髓母细胞瘤中p27表达有不同水平的减低,p27表达的降低及p27在细胞质呈阳性表达是人髓母细胞瘤的危险因素,p27在人髓母细胞瘤中具有预后判断意义的最佳截取值为阳性表达率60%。
Objective To d et ect the expression and localization of p27in human medulloblastoma and its pr ognostic value.Methods Use streptavidin-peroxidase immunohistochemic method to detect the expression of p27and adopt confocal technique to verify its loc alization in57archival medulloblastoma specimens,make the survival analysis with the follow-up materials.Results p27was decreased in medulloblastoma wi th the positive expression ratio ranging from32.41%to96.53%,average d by64.37%±18.10%.Kaplan-Meier survival function illustrated tha t there was significant difference between the higher group(≥80%)and the lower group((<80%)(P<0.0001).The optimal cutoff value of p27w as60%.p27was verified with the cytoplasm localization by confocal techniqu e in16children and7adult cases.Kaplan-Meier survival function revealed it h ad significant difference both in general and children group(P<0.05)of p 27cytoplasm localization,while not in adult group(P>0.05).The lower e xpression and the cytoplasm localization of p27were showed as the independent risk factors for human medulloblastoma by cox proportional hazard model analys is,with the relative risk of4.5(95%CI 2.3～6.7)and3.4(95%C I 1.6～4.1)respectively.Conclusions We find that p27expression decre asein in human medulloblastoma.The lower expression and the cytoplasm localizat ion of p27are of independent prognostic value in human medulloblastoma with the optimal cutoff value of60%.
出处
《中华神经外科杂志》
CSCD
北大核心
2003年第4期288-291,共4页
Chinese Journal of Neurosurgery
