摘要
目的 研究用聚砜膜纤维管构建的新型生物人工肝是否能有效支持肝脏功能。方法应用两步胶原酶法分离猪肝细胞 ,构建聚砜膜中空纤维管生物反应器 ,细胞数量 1× 10 10 个 ,与非生物人工肝同期或非同期使用 ,对 12例患者治疗 14例次 ,每次支持时间为 6h ,治疗前后观察患者一般状况并检测血氨、凝血酶原时间和部分肝功能指标。结果 应用生物人工肝治疗后血氨、凝血酶原时间和总胆红素均明显改善 ,治疗 2d后血氨仍为 (10 6± 131) μmol/L ,与治疗前相比较 [(172± 187)μmol/L]差异有显著意义 (P <0 0 5 ) ;治疗后 1个月 ,同期生物人工肝治疗组死亡 1例 ,非同期生物人工肝治疗组死亡 2例 ,患者总存活率 75 % (9/12 )。结论 我们构建的新型生物人工肝可支持急性肝功能衰竭患者的肝功能 ,同期生物人工肝治疗可能优于非同期生物人工肝。
Objective Using polysulfon fibers, a new bioartificial liver was developed. This study was to evaluate the efficacy of this bioartificial liver in the support of a disfunctioned liver. Methods Hepatocytes were procured from swine using Seglen′s methods. The bioartificial liver was constructed based on polysulfon bioreactor with a procurement of 10 10 hepatocytes, and was applied in 12 acute liver failure patients for 14 sessions. Each BAL treatment lasted 6 hours. The general conditions of the patients and the biochemical parameters were evaluated. Results After treatment with bioartificial liver, ammonia, prothrombin time and total bilirubin level significantly decreased (all P <0 05). Two days later, only blood ammonia remained at a significantly low level ( P <0 05). One month after the treatment, 1 case (1/7) in simultaneous treatment group died while in heterochronous treatment group 2 cases (2/5) died. The total mortality rate was 25%.Conclusions The constructed bioartificial liver can sufficiently support liver functions in acute liver failure. The simultaneous HBAL may be better than non simultaneous HBAL.
出处
《中华普通外科杂志》
CSCD
北大核心
2003年第7期391-393,共3页
Chinese Journal of General Surgery
基金
江苏省卫生厅重点项目 (BQ2 0 0 0 2 0 )
南京市科委发展计划项目 (SS2 0 0 0 0 2 )
关键词
生物人工肝
临床应用
肝功能衰竭
人工膜
Liver failure, acute
Liver, artificial
Membranes, artificial
