摘要
目的 研究昆明山海棠 Tripterygium hypoglaucum(Celastraceae) (THH)根部水提液对早幼粒白血病HL- 6 0细胞凋亡的诱导作用及机制。方法 通过特征性的形态学观察 ,Annexin- V标记 ,以及流式细胞仪检测中次G1 峰的形成确定 THH的诱导细胞凋亡作用 ;应用 Western Blotting研究 THH对 c- Myc蛋白表达的影响。结果THH根部水提液可诱导早幼粒白血病 HL - 6 0细胞凋亡。在浓度高于 18μg/ m L时 ,THH对 HL - 6 0细胞的诱导凋亡作用呈现浓度与时间的相关性。在 THH处理 2～ 4 h后 ,癌基因蛋白 c- Myc表达降低 99%以上。结论 THH抑制了 c- Myc蛋白的翻译或后翻译过程 ,从而降低了 c- Myc在细胞周期运转中的作用 。
Object To investigate the effects of the root aqueous extract of Tripterygium hypoglaucum (Levl.) Hutch (Celastraceae) (THH) on human promyelocytic leukemia HL 60 cells and its mechanism. Methods Cell growth and toxicity assays, flow cytometry, confocal fluorescence microscopy and Annexin V labeling, and Western Blotting Assay were used. Results THH caused morphological changes hypodiploid sub G1 cell accumulation and induced dose and time dependent apoptosis in HL 60 cells at concentrations above 18 μg/mL. Western Blotting analysis showed a more than 99% decrease of the nuclear oncoprotein c Myc after 2—4 h of THH treatment. Conclusion THH decreases c Myc protein expression, leading to the deactivation of cell cycle progression pathways and an accumulation of hypodiploid sub G1 cells that eventually enter apoptosis.
出处
《中草药》
CAS
CSCD
北大核心
2003年第7期622-625,共4页
Chinese Traditional and Herbal Drugs
关键词
昆明山海棠
HL—60细胞
凋亡
c—Myc蛋白表达
Tripterygium hypoglaucum (Levl.) Hutch. (Celastraceae) (THH)
HL 60 cells
apoptosis
c Myc protein expression
