摘要
目的 观察胃癌病人外周血TH1 /TH2细胞及其细胞因子水平的变化 ,为肿瘤的免疫治疗提供实验依据。方法 应用ELISPOT法检测TH1 /TH2细胞 ,ELISA法检测细胞因子。结果 胃癌病人TH1细胞及其细胞因子降低 ,TH2细胞及其细胞因子升高 ,TH1 /TH2比值降低。正常人外周血单个核细胞加IL 1 2和抗IL 4单抗组 ,TH1细胞阳性率增加 ,TH2细胞阳性率降低 ,TH1 /TH2比值升高 ;加IL 4和抗IFN γ单抗组TH1细胞阳性率降低 ,TH2细胞阳性率升高 ,TH1 /TH2比值降低 (P <0 .0 5 )。结论 胃癌病人TH1细胞受抑制 ,TH2细胞亢进 ,导致机体的抗肿瘤免疫应答受抑制。利用IL 1 2诱导TH1细胞的增殖 ,有可能恢复TH1
Objective To observe the variety of T H1/T H2 cell paradigm and cytokines of patients with gastric cancer,so as to give evidence for immunotherapy of tumors.Method Enzyme linked immunospot assay (ELISPOT) was used to detect T H1/T H2 cells,and ELISA were used to assay cytokines.Result T H1 cells and their cytokines in gastric cancer were lower, but T H2 cells and their cytokines were higher compared with normal subjects. During the culture of peripheral blood mononuclear cells (PBMC) when IL 12 and anti IL 4 antibody were added to the culture medium, the positive percentage of T H1 cells were raised while T H2 cells decreased.When IL 4 and anti IFN γ antibody were added,T H1 cells were lower but T H2 cells were higher.Conclusion T H1 cells of patients with gastric cancer were inhibited, which caused inhibited cellular immunity in the patients.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2003年第4期214-216,共3页
Chinese Journal of Clinical Laboratory Science
