摘要
目的 观察BAK和BCL-XL在正常培养和经柔红霉素处理后的人视网膜色素上皮(retinal pigment epithelium,RPE)细胞中的表达。方法 人RPE细胞培养液中加入终浓度为200μg·L^(-1)柔红霉素作用12h,更换新鲜培养液继续培养24h后,运用抗BAK和BCL-XL的多克隆抗体进行免疫细胞化学染色,观察2种基因在RPE细胞中表达的变化。结果 BAK和BCL-XL在正常RPE细胞中均存在表达,但前者的染色强度大于后者(P<0.05),2者光密度分别为56.4±5.7和32.4±4.7,经柔红霉素处理后,RPE细胞BAK的染色强度增加(P<0.05),而BCL-XL的染色强度不变(P>0.05),其光密度值分别为79.2±6.5和34.7±3.9。结论 BAK和BCL-XL在人RPE细胞中存在表达。柔红霉素可以促进BAK的表达,这可能是其诱导RPE细胞凋亡的机理之一。
Objective To study the expression of BAK and BCL-XL in normal and daunorubicin treated human RPE cells. Methods Normal RPE cells were treated with 200fig L-1 daunoubicin for 12 hours, the cells were incubated for an additional 24 hours after changing fresh medium, the expression of BAK and BCL-XL were monitored by immunocytochemical staining with anti-human BAK and BCL-XL antibodies. Results BAK and BCL-XL can be detected in normal human RPE cells, and the staining density of Bak was stronger than that of BCL-XL (P < 0. 05), the OD values were 56. 4 + 5. 7 and 32. 4 + 4. 7 respectively. After treated with daunorubicin, staining density of BAK was increased significantly(P<0.05), while that of BCL-XL had no change (P>0.05), the OD values of BAK and BCL-XL were 79.2 + 6.5 and 34. 7 + 3.9 respectively. Conclusion Normal human RPE cells can express BAK and BCL-XL. Daunorubicin may induce apoptosis of cultured human RPE cells by increasing the expression of BAK.
出处
《眼科新进展》
CAS
2003年第4期233-235,共3页
Recent Advances in Ophthalmology
基金
国家自然科学基金(编号:39700154)~~
