摘要
用透射电镜和扫描电镜技术,对大鼠在动情周期中卵巢颗粒细胞的超微结构进行了超薄切片、割断扫描及冷冻复型观察。结果表明,从早期生长卵泡到成熟卵泡,颗粒细胞的核、细胞器及细胞连接均有改变,并发现颗粒细胞在排卵前已发生黄体化,胞质内出现管状嵴线粒体,滑面内质网(SER)广泛发育,出现电子密度较高的类脂滴。扫描电镜下可见细胞表面高度皱褶并伸出球形突起,细胞表面浮有絮状物。与动情期末(排卵后)功能黄体的颗粒细胞相比,明显不同,尤其是颗粒黄体细胞向细胞间隙突出大量球形泡,部分泡已破裂,并皱缩变扁。表明动情前期末颗粒细胞已开始合成并分泌类固醇激素,动情期末颗粒黄体细胞大量分泌类固醇激素。扫描电镜下所见的微顶浆分泌方式可能是大鼠卵巢类固醇激素分泌方式之一。
Ultrastructures of rat ovarian granulosa cells (GC) during the estrous cycle were observed by electron microscope. The nuclei, cell organelles and intercellular junctions change from early growing follicles to mature follicles. The mitochondrial cristae vary from a lamelliform to tubulovesicular, SER and lipid inclusions accumulate in the cytoplasm. All these changes differ from that of the granulosa luteal cells (GLC). Under SEM, the surface of GC highly wrinkled, and a great number of cloudets are present on it in the late proestrous. In the late proestrous, abundant vesicles appear on the surface of GLC, some of the vesicles burst and the plasmalemmas sink after the contents set free. It suggests that before ovulation the GC may synthesize and start to secrete steroid hormones (progesterone). The secretion activity reachs the maximum in late oestrous. It might be a way to release the steroids hormones by microapocrine secretion in rat ovary.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第3期320-324,共5页
Acta Anatomica Sinica
关键词
颗粒细胞
黄体化
微顶浆分泌
大鼠
Granulosa cells
Luteinization
Microapocrine secretion
Ultrastructure
Rat
