摘要
用精子头部银染法,观察雷公藤多甙及其单体T4、T13、棉酚和微波透热睾丸等抗雄性生育因子,对精子头部异常发生率的影响,以评价其对精子头部异常发生率和对生殖细胞的可能作用。实验表明,雷公藤多甙,单体T13、微波透热睾丸可使精子头部异常发生率明显增加;而棉酚及雷公藤单体T4则无明显增加。对睾丸滴片及睾丸切片的观察也与上述结果相符,表明雷公藤多甙、雷公藤单体T13、微波透热睾丸可能导致生殖细胞产生遗传效应,不宜发展为男性抗生育因子。而棉酚、雷公藤单体T4(雷藤氯内酯醇)则无明显生殖细胞的遗传效应,值得深入研究。
Mutagenesis of several male contraceptives in sperm bead anomalies was investigated. Results show that glycosides of tripterygium wilfordii hook (GTW) and its monomer T13, microwave induce sperm head anomalies. However, gossypol and monomer T4 and GTW do not induce sperm head anomalies. Adult male mice and rats were given orally GTW, monomer T4, T13 and gossypol. These chemical agents were delivered in 1% methylcellulose. Result indicated that frequency of abnormal sperm heads in GTW, T13 groups were significantly increased, while frequency of abnormal sperm heads in T4 and gossypol-treated animals were similar to that of normal controls. When male mice were exposed to microwaves of 0.5 kW for 1-2 min, for five weeks abnormal shape of spermatozoa could be found.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第3期290-293,共4页
Acta Anatomica Sinica
关键词
诱变
精子头部异常
雷公藤多甙
Clastogenicity
Sperm head anomalies
Glycosides of tripterygium wilfordii hook
Monomer T4
Monomer T13
Microwave
