摘要
本文报道兔网织红细胞5srRNA进入小鼠骨髓瘤细胞SP2/0核内,明显地抑制了核内DNA的合成。进而从兔网织红细胞、大鼠胚肝及它们的成熟红细胞,分离提取rRNA,以琼脂糖电泳分析不同发育期红细胞5srRNA、18srRNA、28srRNA组分相对量的变化,又发现5srRNA量的变化与红细胞终末分化期排核过程呈相对应关系。这些结果表明,哺乳动物红细胞5srRNA可能具有抑制核内DNA合成、终止细胞增殖、导致红细胞进行终末分化和排核的作用。
The present study reported that 5srRNA from reticulocytes of rabbit could pass into the nucleus of mouse myeloma cells SP2/0,and in the mean time DNA synthesis and cells division were markedly suppressed.
In a separate experiment, the rRNA was extracted from embryonic liver and erythrocytes of rabbit or rat and analysed by agarose electrophoresis method. The result indicated that the amount of 5srRNA in various period of the development of erythrocyte is changed along with denucleation process. Thus it is likely that 5srRNA of mammalian erythrocyte plays a role in reversing malignant phenotype of tumor cells and promoting denucleation of mammalian erythrocyte through inhibiting DNA synthesis.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第2期159-163,共5页
Acta Anatomica Sinica
基金
国家科委协调攻关局基金
关键词
DNA合成
癌细胞逆转
红细胞
排核
5srRNA
DNA synthesis
Denucleation
Cancer cells reversion
