摘要
本文报道对15例人正常肝组织微体的立体定量研究,以及过氧化氢酶(CAT酶)、葡萄糖-6-磷酸酶(G-6-P酶)、焦磷酸硫胺素酶(TPP酶)、胞嘧啶单核苷酸酶(CMP酶)细胞化学反应的观察。人肝细胞的微体大多呈椭圆形,内含均匀细颗粒状物,单层膜包绕,在胞浆中散在。微体的体密度为1.33±0.38％,数密度为9.76±1.63个/100μm^3,线粒体/微体的数量比为6.22±1.17。酶细胞化学反应的研究发现,内质网腔与微体未见相通,与微体相连的类似滑面内质网的膜结构,呈G-6-P酶细胞化学反应阴性,表明此膜结构不是内质网,可能属于微体的膜系统。高尔基体与微体在结构上未观察到移行关系。而一些微体出现分裂、芽生的现象。此结果表明,新的微体可能由细胞中原有的微体分裂产生。
Peroxisomes (microbodies) were studied with CATase, G-6-Pase, TPPase, CMPase cytochemistry and stereology by electron microscopy in 15 cases of normal human liver specimens. In human hepatocytes peroxisomes are ellipsoid or round intracellular bodies. They contain homogeneous fine granular matrix, and surrounded by unit membrane, and distribute randomly in the cytoplasm. By stereological analysis, the peroxisomes accounted for 1.33±0.38% of the cytoplasmic volume, their numerical density in spase was 9.76±1.63/100 μm^3 cytoplasm, and the ratio of mitochondria to peroxisomes in quantity was 6.22±1.17. The result of G-6-Pase cytochemistry showed no luminal connection between endoplasmic reticulum and peroxisomes. The membrane similar to smooth endoplasmic reticulum seem to be connected with peroxisomes which was G-6-Pase negative, hence such membrane may be peroxisomal membrane system rather than smooth endoplasmic reticulum. The TPPase cytochemistry showed that peroxisomes did not connect with the Golgi complex structurally, and the Golgi complex may not be involved in the biogenesis of peroxisomes. Some figures resembled fission and budding of peroxisomes were viewed, and this finding suggested that new peroxisomes formed probably by division of preexisting peroxisomes.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第2期185-190,共6页
Acta Anatomica Sinica
关键词
微体
超微结构
立体定量
肝细胞
Peroxisome (Microbody)
Ultrastructure
Stereology
Ultracytochemistry
Hepatocyte
Human
