摘要
本文用免疫组织化学PAP法显示SD大鼠胃窦部含5-HT的细胞,并用Weibel体视学方法对其进行定量分析。结果如下:大鼠胃窦部免疫反应阳性细胞仅存在于腺上皮和胃小凹上皮中。该细胞在胃窦粘膜中的体积密度(V_v)为0.0038±0.0004,面数密度(N_A)为42.86±3.20个/mm^2,数密度(N_V)为2627.11±200.42个/mm^3。该细胞的分布有明显的区域性差异。从胃小弯至胃大弯部,细胞密度逐渐降低。小弯区密度最高,两侧壁密度次之;胃大弯区密度最低。大部分5-HT免疫反应阳性细胞位于粘膜深1/3段,该段密度最高,粘膜中1/3段密度次之,粘膜浅1/3段密度最低。此外,本文还对5-HT免疫反应阳性细胞的形态,进行了较详细的观察。
The cells containing 5-hydroxytryptamine (5-HT) in the antrum of the SD rat's stomach were demonstrated by means of Sternberger's PAP immunohistochemical method and quantitative analysis were performed by means of Weibel's stereological method. The results were as follow: The 5-HT immunoreactive cells in the antrum of the rat's stomach were found only in the surface epithelium and the glandular epithelium of the mucosa. The volume density (V_v) was 0.0038±0.0004, the surface density (N_A) was 42.86+3.20 cells/mm^2 and the numerical density (N_v) was 2627.11±200.42 cells/mm^3. The distribution of 5-HT reactive cells showed an obvious regional difference. From the lesser curvature to the greater curvature of stomach, the cell density decreased gradually. The cell density was the highest in the lesser curvature: N_A was 59.96±3.48 cells/mm^2, Nv was 3729.23±216.89 cells/mm^3; and was second high in the two side walls: N_A was 42.39±3.48 cells/mm^2, Nv was 2647.18+216.57 cells/mm^3; and was lowest in the greater curvature: N_A was 29.39± 4.49 cells/mm^2, N_v was 1843.00±280.09 cells/mm^3. Most of the 5-HT immunoreaclive cells were found in the basal third section of the mucosa and the cell density was the highest. N_A was 92.33±6.92 cell/mm^2,N_v was 5336.28±410.22 cells/ mm^3; in the middle one third of the mucosa, the cell density came to next: N_A was 27.69±2.38 cells/mm^2, N_A was 1708.68±146.65 cells/mm^3; and in the superficial third section of the mucosa, the cell density was the lowest: N_A was 7.29±0.53 cells/mm^2, N_v was 457.00±35.44 cells/mm^3. In addition, a detailed observation on the morphology of the 5-HT immunoreactive cells was also undertaken.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第2期200-203,共4页
Acta Anatomica Sinica
基金
国家自然科学基金
关键词
胃窦
粘膜
免疫组织化学
大鼠
细胞
Antrum of stomach
Mucosa
5-HT
Immunohistochemistry
Stereology
Rats
