摘要
应用RNA打点杂交方法,对24例急性非淋巴细胞白血病(ANLL)患者外周血或骨髓细胞及10名正常骨髓细胞中C-myc,C-H-ras的转录水平进行了分析。并在此基础上探讨了小剂量阿糖胞苷和小剂量三尖杉酯碱联合应用,维甲酸诱导分化后白血病细胞C-myc基因表达的变化。结果显示:(1)ANLL患者外周血或骨髓细胞中C-myc基因,C-H-ras基因表达阳性例数及表达强度明显高于正常骨髓细胞。(2)在部分ANLL患者中,C-myc、基因C-H-ras基因同时表达。(3)小剂量阿糖胞苷和小剂量三尖杉酯碱联合应用以及维甲酸均能使部分在体外培养的ANLL细胞的癌基因C-myc表达减低。
RNA dot blot hybridization was employed to investigate the alterations in C-myc, C-H-ras transcript levels in bone marrow and peripheral blood cells from 24 patients with acute nonlymphocytic leukemia. 10 cases of normal bone marrow cells were used as control. We found that the positive rates and intensities of expression of C-myc and C-H-ras in acute nonlymphocytic leukemia were hipher than those of normal control. The C-myc and C-H-ras expressed simultaneously in 10 patients. After having been cultured 5 days with RA and low dose Ara-C combined with low dose Harr, the C-myc transcipt level of C-myc in acute nonlymphocytic leukemia was decreased obviously.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1992年第5期345-348,共4页
Medical Journal of Chinese People's Liberation Army
关键词
基因表达
癌基因
白血病
Gene expression
Oncogene
Acute nonlymphocytic leukemia
