摘要
介绍了1种Ri质粒的快速提取方法,提取的质粒可用于目的基因的扩增,应用rolC基因的特异性引物,扩增出了正确序列的rolC基因0 87kb片段,并利用pGEM-T载体对此片段进行了克隆。
A procedure for rapid extraction of Ri plasmid was developed and a 087 kb fragment of rolC gene was amplified by PCR via primer of rolC gene, and the fragment was cloned into vector pGEM-T The result of sequencing showed that the fragment was exactly rolC gene of TDNA of Ri plasmid
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2003年第3期263-265,共3页
Journal of Jilin Agricultural University
基金
吉林省科学技术厅资助项目(200000054)
