摘要
目的 建立对非清髓性造血干细胞移植术后造血嵌合体的 STR- PCR定量检测技术 ,初步探讨该技术在监测非清髓性造血干细胞移植术后植入状态中的应用。方法 对 3例非清髓性异基因造血干细胞移植术后的患者序列血样 ,在可区分供受者基因的 STR位点上 ,应用凝胶图像分析技术进行供受者特异性等位基因的定量分析。结果 定量分析结果与 DNA电泳条带法的定性分析结果 ,以及临床移植效果一致。在电泳条带已表现为供者源造血时 ,通过定量分析仍可检测出受者特异性等位基因。结论 STR- PCR定量分析技术较单纯电泳鉴定植入可观察到更为细微的变化 。
Objective To establish a method for quantitative analysis of mixed hematopoietic chimerism (MC) based on STR loci. Methods Using STR technique combined with PCR, we analyzed the peripheral blood samples collected from 3 nonmyeloablative stem cell transplantation recipients every 15 days after transplantation. Results The quantitative results coincided with the qualitative results of DNA electrophoresis and with the clinical outcomes. When the result of electrophoresis showed only donor's allele type we could still find out the recipient's specific allele. Conclusion The quantitative analysis using STR-PCR can detect more subtle changes in the evidence of engraftment. It is a more efficient method for detect the hematopoietic chimerism post transplantation.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2003年第3期568-570,573,共4页
Journal of Sichuan University(Medical Sciences)
基金
四川省学术和技术带头人培养基金 (课题号 498990 5 )资助
