摘要
目的 进一步探讨石棉理化特性在致突变 /致癌中的作用。方法 用免疫组织化学方法检测石棉悬液和浸泡过滤液对 V79细胞的 P5 3蛋白表达的影响。结果 石棉悬液和石棉浸泡过滤液处理组与阴性对照组比较 ,p5 3基因编码的 P5 3蛋白平均灰度下降 ,具有显著性差异 ( P<0 .0 5 ) ,表明 P5 3蛋白异常表达。出现双核或多核细胞的胞核有明显的 P5 3蛋白异常表达。结论 P5 3蛋白异常累积与石棉暴露有关 ,且对分裂期细胞 P5
Objective To explore the effects of physical and chemical properties of asbestos on mutagenesis/carcinogenesis. Methods The P53 protein expression in V 79 cells exposed to asbestos suspension and filtrate was studied using immunohistochemical method. Results After asbestos suspension and filtrate treatment of V 79 cells, the value of hue for indicating P53 protein expression was significantly lowered, and such abnormal expression was especially observed in binucleate cells and polykaryocytes. Conclusion The above data demonstrated that abnormal accumulation of P53 protein in V 79 cells was related to asbestos exposure and that asbestos suspension and filtrate had even greater effect on the cells at the segmentation period.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2003年第3期516-518,共3页
Journal of Sichuan University(Medical Sciences)
