摘要
目的 探讨精索上神经对精子发生的调控作用。方法 SD大鼠分为实验组和假手术组 ,实验组分离并切除睾丸精索上神经 ,假手术组分离但不切除该神经 ,运用光镜和电镜观察睾丸组织病理学变化 ;脱氧核糖核酸末端转移酶介导的 d U TP缺口末端标记技术 ( TUNEL)检测生殖细胞凋亡。结果 切除精索上神经导致生精上皮萎缩、生精功能退化 ,电镜下生殖细胞内染色质出现浓缩、断裂等改变 ,TUNEL 进一步显示切除精索上神经诱发精原细胞和间质细胞大量凋亡。结论 精索上神经参与对精子发生的调控 。
Objective To evaluate the role of superior spermatic nerve in the regulation of spermatogenesis. Methods Mature SD rats (350-375 g) received bilateral surgical removal of the superior spermatic nerve(SSN). The animals were killed 1 and 2 months after operation and were studied in comparison with the SD rats of the sham operation group. H.E. staining was employed in observing spermatogenesis, and transmission electron microscopy and terminal deoxynuleotidyl tansferase-mediated dUTP nick end-labeling (TUNEL) were used to detect apoptosis. Results No pathological changes were observed 1 month after operation. Two months after SSN denervation, spermatogenesis arrest was seen in the seminiferous tubules in which only Sertoli cells or Sertoli cells and a few spermatogonia remained. Chromosome condensation and breakage in germ cells were observed under transmission electron microscope in denervated testes. TUNEL showed that SSN denervation induced apoptosis of spermatogonia and Leydig cells significantly. Conclusion The superior spermatic nerve seems to be involved in the regulation of spermatogenesis. Further study is worth doing to clarify the possible mechanism of action.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2003年第3期462-464,共3页
Journal of Sichuan University(Medical Sciences)
