摘要
芦笋鲜嫩幼笋茎作外植体。在MS+2.4-D0.1 mg/L+Kt0.1 mg/L的培养基中。能100％诱导出愈伤组织,呈乳白色。质地疏松。在相同的培养基上,能反复多次切割进行继代培养。当转移到MS+IAA0.05ms/L+6BA0.1mg/L培养基中,能高频率地分化出苗。当苗高1～5 cm时,转移到MS+IAA1mg/L培养基上。15～20 d即可长出新根,完成完整再生植株培养主过程。当将已分化出苗的愈伤组织转移到MS+IAA0.5mg/L+6BA1mg/L培养基上,小苗能茁壮成长,愈伤组织部分可以不断扩大生长,并不断分化出新的芽苗,已分化的小苗可长出粗壮新根。如将其中愈伤组织转移到同一培养基上,仍然保持上述能力。
Callulses can be induced from the tender asparagus stalk as the explant by 100 per cent on MS medium supplemented with 2,4-D 0.1mg/L and kt 0.1mg/L. The calluses are milky white and loose. On the same medium, they can be cut many times for successive culture. When transfered to MS medium supplemented with IAA 0.05mg/ L and 6-BA 0.1mg/L. they will differentiate seedlings with very high frequency. When the seedlings grow up to 1～5cm, they are transfered onto MS medium supplemented with IAA 1mg/L. 15～20 days later, new roots will develop. When the calluses from which seedlings have been differentiated are transfered on to MS medium supplemented with IAA 0.5mg/L and 6～3A 1 mg / L, they will expand and differentiate new seedlings continuously. On this medium, the seedlings grow in flourish and produce sturdy new roots.
出处
《江西农业大学学报》
CAS
CSCD
1992年第4期329-332,共4页
Acta Agriculturae Universitatis Jiangxiensis
关键词
组织培养
石刁柏
Asparagus
Explant
Tissue culture
Successive culture
