摘要
为探讨肉种鸡免疫禽脑脊髓炎 (AE)弱毒疫苗的抗体变化规律及其与后裔雏鸡对禽脑脊髓炎病毒 (AEV)易感性的相互关系 ,本研究用琼脂扩散试验对 14周龄免疫禽脑脊髓炎 (AE)弱毒疫苗的 5群艾维茵父母代肉种鸡 ,其中 1群于 34周龄加免AE油乳剂灭活苗 ,从 2 0至 6 0周龄对其AE抗体进行动态监测。结果发现 :1)单免弱毒疫苗的 4群种鸡 ,其AE抗体阳性率呈双低谷曲线变化规律 ,32周龄之前较高 ,为 95 %～ 83.4 % ;36～ 4 0周龄降至 6 7.5 %～ 71.1% ,最低可至 5 6 .7% ;4 4周龄转而升至 80 %以上 ,4 8～ 5 2周龄再度降至 5 9.1%～ 6 7.5 % ;5 6周龄以后又呈上升 ( 84 .2 % )。 2 )AE抗体低谷期间种鸡产蛋率明显降低 ,其后裔雏鸡AE母源抗体明显降低 ,而且衰减快。 14日龄颈部皮下注射AEVVanRoekel,5 0 %的雏鸡在攻毒后 13d发生AE。有些雏鸡在 14～ 2 1日龄自然发生AE。 3)加免AE油乳剂灭活苗可避免种鸡AE抗体低谷期的出现。以上结果表明 ,在我国养鸡实际生产中种鸡开产前免疫 1次AE弱毒疫苗 ,不足以为整个产蛋期提供有效保护 ,AE弱毒疫苗和灭活油苗联合应用是预防AE的有效措施。
Five broiler breeder flocks were inoculated with live Avian Encephalomyelitis (AE) virus vaccine by Wing-Web administration at 14 weeks of age. The Fifth flock was inoculated with killed AEV vaccine once more at 34 weeks of age. AE-antibody of the five flocks were detected dynamically with Agar gel precipitation (AGP) test. The results showed that the AE antibody of the 4 breeder flocks inoculated with live AEV vaccine changed in a 'double vallys'pattern: the AGP-positive percentage was the highest,being 95%～83.4% in the samples drawn before 32 weeks of age, and decreased to 67.5%～71.7% at 36～40 weeks of age. But the positive percentage increased again over 80% at 44 weeks of age, and then decreased to 59.1%～67.5% at 48～52 weeks of age, finally increased to 84.5% after 56 weeks of age. The egg production dropped during the 'vallys', which might be resulted from the infection of the AEV field strains. While the AE antibody level of the fifth flock, inoculated with killed AEV vaccine once more, kept at a high level. There was a positive relation between the maternal antibody of one day age progeny chickens and the antibody of breeder flocks. The maternal antibody of the progeny chicken in the 'vally' was the lowest, which resulted in the AE in some progeny chickens at 14～21 days of age.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2003年第3期103-106,共4页
Journal of China Agricultural University
