摘要
实验设置5个处理组,研究瘤胃微生物不同种群对小麦秸的降解作用。结果发现:瘤胃细菌在整体上对纤维素性物质的降解力强;瘤胃真菌也能降解部分粗纤维类物质,但作用较小;瘤胃细菌和真菌在降解中具有一定的互作关系。体外干物质降解率、总挥发性脂肪酸、总气体产量、总甲烷气体产量、羧甲基纤维素酶活力可以作为评定瘤胃微生物降解活力的指标。
The role of anaerobic rumen bacterial and fungi in vitro wheat straw degradation was determined with five treatments. Forage used as substrates for rumen microorganism was wheat straw; Inoculum was rumen fluid samples from a Huanghuai white goat. Treatments were whole rumen fluid(WRF),WRF plus streptomycin(02 mg/mL of rumen fluid)and penicillin(125 mg/mL of rumen fluid),WRF plus cycloheximide(05 mg/mL of rumen fluid),WRF plus streptomycin (02 mg/mL of rumen fluid) ,penicillin(125 mg/mL of rumen fluid),and cycloheximide (05 mg/mL of rumen fluid) and McDougall buffer.The results showed that the most active fiber digesting population in rumen was the bacteria.Fiber degradation by anaerobic fungi was significantly less.Besides that,the parameters of in vitro dry materials digestion ratio,total gas production, methane production,total volatile fatty acids production, activity of carboxymethylcelluosase can be used to assess degradation ability of rumen microbiology.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2003年第3期285-288,共4页
Journal of Anhui Agricultural University
关键词
瘤胃微生物种群
小麦秸
体外降解率
rumen microbiology population
wheat straw
in vitro degradation
