摘要
目的 :观察气滞血瘀、气虚血瘀与血小板活化分子α-颗粒膜蛋白 (CD6 2 p)、溶酶体完整膜蛋白 (CD6 3)、淋巴细胞巨噬细胞分化抗原 - 1(CD11b/ CD18)细胞黏附分子表达的关系。方法 :采用单克隆抗体和流式细胞仪技术 ,研究不同病种气滞血瘀和气虚血瘀患者血小板活化分子 CD6 2 p、CD6 3及淋巴细胞膜 CD11b/ CD18的表达。结果 :气滞血瘀、气虚血瘀组患者的 CD6 2 p、CD6 3及 CD11b/ CD18表达量皆显著高于正常对照组 (P <0 .0 1) ;气滞血瘀组患者的 CD6 2 p、CD6 3及 CD11b/ CD18表达量也皆显著高于气虚血瘀组患者 (P <0 .0 5 ) ;CD6 2 p与 CD6 3、CD11b/ CD18表达量明显相关 (r =0 .74 0 ,P <0 .0 1;r =0 .781,P <0 .0 1)。结论 :血瘀证患者的 CD6 2 p、CD6 3及CD11b/ CD18表达量明显增加 。
Objective:To observe the correlation between platelet activation molecules α granule membrane glycoprotein(CD62p),lysosomal integral membrane protein(CD63),macrophage antigen compound(CD11b/CD18) on lymphocyte and two TCM syndromes,blood stasis due to Qi stagnation(BSQS) and blood stasis due to Qi defiency(BSQD).Methods:Expressions of platelet activating molecules CD62p,CD63 and CD11b on lymphocyte in patients with BSQS or BSQD were determined quantiatively with flow cytometry and specific monoclonal antibody against activated platelet.Results:Expressions of CD62p,CD63 and CD11b in the above patients are higher than those in the normal control group significantly(P<0.01).Expressions of CD62p,CD63 and CD11b in the BSQS group were higher than those in the BSQD(P<0.05).A positive correlation was shown between CD62p and CD63(r=0.744,P<0.01)and that between CD62p and CD11b(r=0.781,P<0.01).Conclusion:Expressions of CD62p,CD63 and CD11b in blood stasis patients are higher than those in normal people,and the increased degree of expressions might be related to the types of blood stasis.
出处
《山东中医药大学学报》
2003年第4期274-276,共3页
Journal of Shandong University of Traditional Chinese Medicine
基金
山东省中医管理局课题 ( 2 0 0 1-2 -64 )
