摘要
目的 :观察青藤碱对佐剂性关节炎大鼠滑膜细胞核因子 κB(NF κB)活性及TNF αmRNA、IL 1βmRNA、IL 10mRNA的影响 ,阐明该药治疗类风湿关节炎的可能机制。方法 :以佐剂性关节炎大鼠为模型 ,收集滑膜细胞 ,采用电泳迁移率改变分析法检测NF κB的活性 ,反转录 PCR检测TNF αmRNA、IL 1βmRNA、IL 10mRNA的表达。 结果 :佐剂性关节炎大鼠滑膜细胞内NF κB活性与TNF αmRNA、IL 1βmRNA、IL 10mRNA的表达均显著升高 ,青藤碱在一定的浓度范围内呈浓度依赖性抑制NF κB活性与TNF αmRNA、IL 1βmRNA的表达 ,而对IL 10mRNA的抑制效应与浓度无关。 结论 :青藤碱可能通过抑制NF κB活性而降低滑膜细胞内TNF αmRNA及IL 1βmRNA的表达。
Objective:To survey the effect of sinomenine (SIN) on the activity of nuclear factor κB (NF κB) of and the expression of TNF α mRNA, IL 1β mRNA as well as IL 10 mRNA by synoviocytes in a rat model of adjuvant arthritis(AA) in order to clarify the possible mechanism underlying the therapeutic effectiveness of the drug in the treatment of rheumatoid arthritis. Methods:The experimental rat model of adjuvant arthritis(AA) was used and synoviocytes were collected. The NF κB activity was measured by electrophoretic mobility shift assay (EMSA) and mRNAs of TNF α, IL 1β and IL 10 were detected with reverse transcriptase PCR(RT PCR). Results:The NF κB activity of and expression of mRNAs of TNF α, IL 1β and IL 10 by synoviocytes were markedly increased. In a definite range of concentration, SIN was shown to inhibit the NF κB activity and the expression of the mRNAs of TNF α and IL 1β in a concentration dependent manner. However, inhibition of IL 10 mRNA was not dependent on the SIN concentration. Conclusions:It seems possible that SIN decreases the mRNA expression of TNF α and IL 1β by inhibiting the NF κB activity of synoviocytes.
出处
《医药导报》
CAS
2003年第8期519-521,共3页
Herald of Medicine
