摘要
以牛儿基牛儿基二磷酸为前体的紫杉醇生物合成大约有 2 0步酶促反应 ,其反应过程已基本阐明 ,近一半的相关酶基因已得到克隆与表达。综述了编码参与紫杉醇生物合成的紫杉二烯合酶、紫杉二烯 5α 羟化酶、紫杉烷 1 0 β 羟化酶、紫杉烷 1 3α 羟化酶、紫杉二烯 5α 醇 O 乙酰基转移酶、紫杉烷 2α 苯甲酰转移酶、去乙酰基巴卡亭Ⅲ 1 0 β O 乙酰转移酶、3 氨基 3 苯基丙酰转移酶和 3 N 去苯甲酰 2 脱氧紫杉醇 苯甲酰转移酶等 9个酶基因的克隆和表达方面的研究情况 ,并指出随着紫杉醇生物合成的分子生物学研究的不断深入 。
The pathway of Taxol biosynthesis from geranylgeranyl diphosphate consists of about 20 enzymatic steps,which has been elucidated approximately.This paper summarized the cloning and expression of the 9 genes encoding taxadiene synthase,cytochrome P450 taxdien 5 alpha hydroxylase,cytochrome P450 taxane10 beta hydroxylase,cytochrome P450 taxane 13 alpha hydroxylase,taxadien 5 alpha ol O acetyl transferase,taxane 2 alpha O benzoyltransferase,10 deacetylbaccatin III 10 O acetyl transferase,3 amino 3 phenylpropanoyltransferase and 3′ N debenzoyl 2′ deoxytaxol N benzoyltransferase,respectively.The production of Taxol on large scale by biotechnology will become true recently with the development of study on Taxol biosynthesis in molecular biology.
出处
《中国生物工程杂志》
CAS
CSCD
2003年第6期36-40,共5页
China Biotechnology
基金
四川省应用基础研究重点项目 (0 1sy0 5 1 3 2 )
