摘要
目的 :观察川芎嗪对体外培养的家兔关节软骨细胞的Bcl 2蛋白表达及凋亡的影响。方法 :取家兔的关节软骨 ,将软骨细胞分离后进行体外培养。传至第二代后重新制成软骨细胞悬液 ,按 2 .5× 1 0 5浓度接种于载玻片上 ,每片 1 .8ml。分为 2个实验组 ,一组加入川芎嗪注射液 0 .2ml(川芎嗪组 ) ,一组加入等量培养基作为对照组 ,再置入 37℃ ,5 %CO2 培养箱内培养 2 4h后 ,用免疫组化的方法检测各组Bcl 2的表达 ,及用TUNEL法 (脱氧核糖核苷酸末端转移酶介导的缺口末端标记法 )观察各组软骨细胞的凋亡。结果 :①川芎嗪组对体外培养的家兔关节软骨细胞Bcl 2蛋白明显高于对照组 ,差异有显著性 (P <0 .0 1 ) ;②川芎嗪组的软骨细胞凋亡率明显低于对照组 ,差异有显著性 (P <0 .0 1 )。结论
Objective To determine the effect of ligustrazine on the expression of Bcl 2 protein and apoptosis in rabbit articular chondrocytes in monolayer culture.Methods We separated chondrocytes from the rabbit articular cartilage and cultured them in vitro . We divided them into 2 groups.One group was added the injective ligustrazine hydrochloride(LH),and the other was added the same quantity of culture medium as the comparison. The expression of Bcl 2 in every group was examined with immmunohistocytochemistry technique, and the apoptosis was observed with TUNEL. Results The two groups both expressed Bcl 2 protein,but the expression in the LH group was significantly higher than that in the comparison group ( P <0.01). The two groups both had apoptosis, the rate of which in the LH group was significantly lower than that in the comparison group( P <0.01). Conclusion Ligustrazine may repair the cartilaginous lesion.
出处
《湖南医科大学学报》
CSCD
北大核心
2003年第3期224-226,共3页
Bulletin of Hunan Medical University
基金
湖南省卫生厅医药科研基金 (编号 :2 0 3 16)
