摘要
基因表达系列分析 (Serialanalysisofgeneexpression ,SAGE)是一种研究真核细胞表达基因信息的高通量检测技术 ,它能对细胞内所有表达基因进行定性与定量分析。近年来此技术广泛应用于获得表达基因谱的研究 ,并且可以发现新基因信息 ,还可发现基因的靶向定位以及对其它基因的影响 ,明确表达基因的功能。本文就SAGE的原理、实验方案。
Serial analysis of gene expression (SAGE) is a powerful high-throughput experimental technique designed to gain a qualitative and quantitative measure of global gene expression in eukaryotic organisms. The SAGE method is widespread applied in obtaining global profile of gene expression in a given tissue in past years. The tags of SAGE provide evidences for annotation of expressed genes, and roles of target genes in the gene network by regulating other products; its unknown tags also provide evidences for the expression of potential new genes. This review is detailed with the introduction of SAGE in principle, protocol, methodological evolution and retrospective-prospective applications.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第4期315-321,共7页
Immunological Journal
基金
国家自然科学基金资助 (30 2 71199)
关键词
基因表达系列分析
转录组学
基因表达谱
原理
实验方案
Serial analysis of gene expression (SAGE)
Global profile of gene expression
Transcriptome
