摘要
目的测定中国汉族人PLAG1cDNA序列。方法收集中国汉族人新鲜腮腺肿瘤组织标本共3例,新鲜胎盘1例,胎儿肝、肾组织1例,用RT-PCR方法扩增PLAG1cDNA,克隆入pGEM-Teasy载体后(或直接)用双链四色荧光标记法进行序列分析。结果PLAG1编码序列的第1372号碱基存在A→C的点突变,使其编码的第457位氨基酸由苏氨酸(Thr)变为脯氨酸(Pro)。结论编码序列第1372号碱基A→C的点突变,可能是中国汉族人PLAG1的一个多态性位点。
Objective To detect the sequence of PLAG1cDNA in Chinese.Method s Reverse transcription-polymerase chain reaction(RT-PCR)and double-strand DNA seq uencing were used to analyze sequence of PLAG1cDNA in Chinese.3cases of parotid gland tumor(2cases of pleomorphic adenoma,1case of Warthin's tumor)and2cases of human tissues(1case of placenta,1case of fetal liver and kidney)were investigat ed.Results An A→C breakpoint was found in1372base pair(bp)of coding sequence of PLAG1in all5cases.Conclusion The A→C1372bp breakpoint in coding sequence maybe a polymorphic point of PLAG1cDNA in Chinese.
出处
《中国口腔颌面外科杂志》
CAS
2003年第2期99-101,共3页
China Journal of Oral and Maxillofacial Surgery
基金
上海市科委科技基金(02-199)
