摘要
目的 探讨原发性胃癌中SMAD 2杂合性丢失 (LOH )的情况及其与胃癌临床病理学之间的关系。方法 用多聚酶链反应 -单链构相多态性 (PCR SSCP )银染法分析 5 0例原发性胃癌SMAD 2的LOH。结果 D 18S45 0位点发生LOH的频率为 48.9% ( 2 2 /4 5 ) ,D 18S460为 3 3 .3 %( 16/4 8) ;SMAD2发生LOH的频率为 5 5 .1% ( 2 7/4 9)。在 2 5例淋巴结有转移的胃癌中 ,18例( 72 % )发生SMAD 2LOH ;2 4例无转移者中 ,9例 ( 3 7.5 % )发生SMAD 2LOH (P <0 .0 5 )。在 2 4例TNMⅢ期和Ⅳ期原发性胃癌中 ,有 17例 ( 70 .8% )发生SMAD2LOH ;而 2 5例TNMⅠ期和Ⅱ期原发性胃癌中 ,仅 10例 ( 4 0 .0 % )发生SMAD 2LOH (P <0 .0 5 )。SMAD2与患者的性别 ,年龄 ,肿瘤的部位、大小、组织学分级 ,Lauren及Borrmann分型均无明显相关 (P >0 .0 5 )。结论 SMAD2的LOH可能在胃癌的发展和转移中起重要作用 ,是胃癌发展后期的重要分子事件。
Objective To investigate the role of loss of heterozygosity (LOH) of SMAD2 and its relationship with clinicopathological parameters of primary gastric carcinoma. Methods Fifty cases of primary gastric carcinoma were monitored by polymerase chain reaction -single strand conformation polymorphism (PCR- SSCP) and silver staining to detect SMAD2 LOH. Results The incidence of LOH was 40.0%(22/45) at D18S450 and 33.3%(16/48)at D18S460 . LOH occurred in SMAD2 was 55.1%(27/49).The rate of SMAD2 LOH was 72.0% (18/25) in primary gastric carcinoma with lymph node metastasis , which was significantly higher than that in without lymph node metastasis (P<0.05). The prevalence of SMAD2 LOH in TNM stage Ⅲ and Ⅳ (17/24, 70.8%) was significantly higher than that in TNM stage Ⅰand Ⅱ (10/25, 40%) (P<0.05). Conclusions The SMAD2 LOH may promote the progression and matastasis of gastric carcinoma, and is an important event in the late stage of primary gastric carcinoma.
出处
《中国普通外科杂志》
CAS
CSCD
2003年第7期500-504,共5页
China Journal of General Surgery
基金
上海市医学领先专业 (胃肠道肿瘤生物学特性及综合治疗的基础与临床研究
98- Ⅲ -0 0 5)
上海市实体瘤攻关项目 (98 ZD 0 0 1 )
