摘要
目的 研究小鼠免疫接种乙型肝炎病毒 (HBV)表面 S抗原疫苗 (S- HBs Ag)后引起的特异性、MHC- 限制性细胞毒 T淋巴细胞 (cytotoxic T lymphocytes,CTL)反应。方法 分别给 BAL B/c(H- 2 d)小鼠腹腔内接种 0～ 6 μg S- HBs Ag,2周后加强 1次 ,4周后分离小鼠脾淋巴 T细胞 ,体外用小鼠 Ld限制的、S- HBs Ag特异性 CTL表位多肽 S2 8- 39- 刺激 ;用特异性多肽 S2 8- 39- 、51 Cr标记的 P815细胞作为靶细胞 ;4 h51 Cr释放实验检测 CTL 反应。结果 0、0 .6 5、1.2 5、2 .5、5 μg组小鼠 ,CTL 特异性释放率分别为 31.2 1%± 9.2 3%、4 2 .36 %± 19.32 %、91.2 1%± 2 2 .97%、6 9.2 5 %± 2 4 .13%、5 1.4 9%± 2 1.6 6 1% ;只接受 1次免疫接种的小鼠 ,其脾淋巴细胞特异性 CTL 特异性释放率分别为 2 7.34%± 14 .2 5 %、32 .2 7%± 15 .35 %、5 6 .2 8%± 2 4 .35 %、4 4 .34%± 18.6 5 %、4 0 .76 %±5 6 %。结论 BAL B/c(H- 2 d )小鼠腹腔内接种 S- HBs Ag引起特异性 CTL 反应 ,加强免疫能够提高 CTL 反应。
OBJECTIVE The hepatitis B virus (HBV) small surface vaccine(S HBsAg) specific MHC class Ⅰ restricted cytotoxic T lymphocytes (CTL) responses induced by immunization with S HBsAg in mice were assayed. METHODS BALB/c (H 2 d) mice were injected intraperitoneally (i.p.) with S HBsAg at doses ranging from 0 to 6 μg and boosted after 2 weeks. Four weeks after the last immunization, spleen cells were restimulated in vitro with specific peptides, which represent a L d restricted CTL epitope of the S protein in BALB/c (H 2 d) mice. The specific cytotoxic reactivity of in vivo primed and in vitro restimulated cells was tested against P815 cells impulsed with specific peptides and labeled with 51 Cr. Four hour release assay of 51 Cr was performed. RESULTS The percentages of specific release of the murine spleen lymphocyte in groups 0, 0.65, 1.25, 2.5, 5 μg were 31.21±9.23% , 42.36±19.32%, 91.21±22.97%, 69.25±24.13%, 51.49±21.66%, respectively. For the mice received only priming vaccination, the percentages of specific release of the mice spleen lymphocyte in groups 0, 0.65, 1.25, 2.5, 5 μg were 27.34±14.25%, 32.27±15.35%, 56.28±24.35%, 44.34±18.65% , 40.76±56.00%, respectively. CONCLUSIONS Injection with S HBsAg efficiently primed MHC Ⅰ restricted CTL response to the S HBsAg in BALB/c (H 2 d) mice, which was enhanced by boosting injection.
出处
《中华医院感染学杂志》
CAS
CSCD
2003年第7期612-615,共4页
Chinese Journal of Nosocomiology
基金
广东省重点科技项目 (99M0 481 1 G)
