摘要
目的 研制华支睾吸虫病金标快速免疫诊断试剂盒 ,评价其敏感性、特异性和现场试用效果。 方法 以华支睾吸虫成虫水溶性抗原、分泌排泄抗原和重组抗原磷酸甘油酸激酶 (PGK)作为诊断试剂 ,制备胶体金免疫层析检测 (Im-munochromatography test,ICT)试剂盒 ,检测患者血清和唾液中抗华支睾吸虫 Ig G,并与常规 EL ISA血清学检测方法和粪便虫卵检查法相比较。 结果 实验室检测临床确诊的华支睾吸虫病人血清中特异的 Ig G,3种抗原的敏感性均为10 0 %。天然抗原除与慢性血吸虫病人血清有较强的交叉反应外 ,与囊虫、包虫、弓形虫病人血清没有交叉反应。重组PGK抗原同慢性血吸虫病人血清也没有交叉反应。用分泌排泄抗原制备的 ICT检测试剂盒在流行区现场检测被调查者的血清和唾液 ,总符合率为 92 .31% ;ICT与 EL ISA血清检测的总符合率为 81.5 4 %。现场获取了 9人的粪便样本 ,其中 4人检出华支睾吸虫卵 ,其血清 ICT和 EL ISA检测均呈强阳性 ,另外未检出虫卵的 5人中 ,1人血清 ICT和 EL ISA均呈阳性 ,另 1人仅血清 EL ISA呈弱阳性。 结论 诊断华支睾吸虫感染的 ICT抗体检测技术 ,尤其是无创性唾液检测技术 ,简便、快速、准确、安全 ,优于血清 EL ISA检测和粪便虫卵检测 ,适用于临床检验和现场大规模流行病?
Objective To develop a rapid colloid-gold labeled immunological diagnosis kit for clonorchiasis, and estimate its sensitivity, specificity, and manifestation in field application. Methods The worm water-soluble antigens, secretory-excretory antigens, and recombinant antigen phosphate glyceric acid kinase (PGK) were used as diagnostic reagents. The sensitivity and specificity of these antigens were estimated in laboratory and field by ICT kits, and ICT was compared with ELISA and microscopy of eggs in stool. Results In laboratory, the positivity of IgG against Clonorchis sinensis in sera from clinically proved clonorchiasis patients was all 100% by 3 ICT kits, the natural antigens showed some cross-reaction with sera from chronic schistosomiasis patients and no cross-reaction with sera from Cysticercosis, Echinococcosis and Toxoplasmosis patients. The recombinant antigen PGK had no cross-reaction with all these sera. In field test, the accordance of sera and saliva assay by ICT was 92.31%, and that of ICT and ELISA was 81.54%; microscopy found C. sinensis eggs in stool samples of 4 persons whose sera were strongly positive for specific IgG by ICT and ELISA. 1 of the other 5 persons negative for eggs was positive for serum IgG by ICT and ELISA, another was only slightly positive for sera IgG by ELISA. Conclusion The way of ICT detecting IgG for diagnosis of clonorchiasis, particularly the no-injury saliva detection, was simple, rapid, accurate, and safe, superior to sera ELISA and microscopy for eggs in stool, suitable for clinical diagnosis and large scale epidemical investigation in field.
出处
《中国寄生虫病防治杂志》
CSCD
2003年第3期152-154,共3页
Chinese Journal of Parasitic Disease Control
基金
广东省医学科技联合攻关项目 (No.2 0 0 2 B31 0 0 5)
广州市重点科技攻关课题 (No.2 0 0 2 2 3- E40 2 2 )。
