摘要
对重组基因工程酵母 (PP NP 1)进行诱导培养 ,然后对诱导表达物进行饱和硫酸铵分级沉淀 ,再对沉淀进行Western印迹 ,证实其为特异的植酸酶 ;再对基因工程菌所产植酸酶进行纯化 ,并对其酶学性质进行了研究。研究结果 :测得其分子量为 70 2kD ;酶作用最适温度为 5 5℃ ;在 pH 2 5～ 5 6范围内植酸酶均具较高的酶活性 ,最适pH为 4 6 ;另外Ca2 + 、牛血清白蛋白、Mn2 + 、Mg2 + 对酶有激活作用 ,而HPO42 、SDS、Cu2 + 、Fe2 + 对酶有抑制作用。
the phyA gene has been expressed as an active enzyme in pichia pastoris The expressed extracellular phytase has been verified by Western blotting analyse,and it is to be a specific product of the phyA gene and a functional enzy me The expressed extracellular phytase was purified by ammonium sulfate precipi tation Its molecular weight is 70 15kD, and optimalpH is 4 6 Its optimal temperature is 55℃ The enzyme can be activated by the Ca 2+ ?moggy serum albumin?Mn 2+ ? Mg 2+ ; inhibited by HPO 2+ 4?SDS?Cu 2+ ?Fe 2+
出处
《微生物学通报》
CAS
CSCD
北大核心
2003年第3期38-41,共4页
Microbiology China
基金
国家科技部"九五"
"十五"国家科技攻关计划资助项目(No9900902)
关键词
基因工程酵母
植酸酶
性质
Gene engineering yeast, Phytase, Characters
