摘要
由土壤中筛选到一能产生新型抗肿瘤抗生素AGPM的藤黄灰链霉菌株 ,应用蛋白质双向电泳方法 ,比较了藤黄灰链霉菌在发酵 2 4h与 72h蛋白质表达的差异 ,发现在发酵 72h有17个蛋白质差异点出现 ,此外还发现一些蛋白质的含量明显高于 2 4h的同种蛋白含量 。
A new strain of Streptomyces regensis was isolated from soil to produce a novel antibiotic AGPM possessing a strong antitumor activity In order to study on the metabolic path of the novel antibiotic AGPM, the protein patterns from the strain of Streptomyces regensis at different culture period were analyzed by using two eimensional polyacrylamide gel electrophoresis Comparing with sample from growth phase, seventeen new protein spots were found in that from antibiotic production phase The results demonstrated that the special proteins might be related with the antibiotic AGPM biosynthesis from Streptomyces regensis
出处
《微生物学通报》
CAS
CSCD
北大核心
2003年第3期6-9,共4页
Microbiology China
基金
国家自然科学基金资助项目 (No 3 0 2 0 0 0 0 3 )
国家高技术研究发展计划项目 ("863"项目 ) (No 2 0 0 1AA2 14 0 81)~~
关键词
藤黄灰链霉菌
AGPM
蛋白质组
二维凝胶电泳
Streptomyces regensis , AGPM, Proteome, Two dimensional gel electrophoresis
