摘要
按张果(1952)的方法,用46℃水浴处理家蚕未受精卵18分钟,获得发育卵,进一步对30多个蚕品种和杂交种,对热处理卵的孵化、生长情况作了比较,发现蚕品种间热处理效果差异大。先后饲养近2万条热处理后代,全是雌蚕,标记基因证实它们的基因型和母本相同,在AcmaypoB(1973)的基础上,将热处理卵发育成雌蛾的未受精卵,再次用热处理继代成功,连续用这种方法继代,已获得几个雌蚕无性繁殖系(clonc),处理卵的孵化率逐代提高,蚕也一代比一代好养,按Stronnikov(1975)方法,用-11℃或-17℃冷冻30分钟,处理了30多个蚕品种或杂交种的未受精卵,获得一些雄蚕,用标记基因作遗传分析,结果表明它们都是纯合体,进一步饲养这种雄蚕,用雄核发育方法继代成功。
According to method of Zhang Guo (1952), the development eggs were obtained by soaking treatment at 46℃of water for 18 minutes. Comparison was done for treated eggs hatching and growth for 30 strains and hybrids. 20 000 offsprings from treated eggs have been reared. They were all female silkworms. It is proved by marker genes that their genotype is the same with that of their mother completely. Heat treatment effect is quite different between strains.
On the basis of Astaurov (1973), non-fertilized eggs of female moth from heal treatment eggs were heated again and it is success in inheriting generation. Several parthenogenesis clones have been formed using this method continuously. Hatching rate of treated eggs is increased gradually and hatched silkworm arc very easy to rearing generation by generation.
According to method of Stronnikov (1975), non-fertilized eggs from more than 30 strains or hybrids were frcczcd for 30 minutes under -11℃ or-17℃. Some male silkworms have been obtained by this method. Genetic analysis by marker gene proved that they are all homozygous. Obtained male silkworms were reared and inheriting generation was succcsful using androgcnesis method.
出处
《蚕业科学》
CAS
CSCD
1989年第4期202-206,共5页
ACTA SERICOLOGICA SINICA
关键词
蚕
染色体
遗传工程
人工单性生殖
Bombyx mori Chromsomc Genetic engineering Parthenogenesis
