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脑缺血再灌注中补体激活的实验研究 被引量：1

Experimental Investigation of Complement Activation in Cerebral Ischemia-Reperfusion
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摘要 目的探讨脑缺血再灌注中的补体激活及其作用。方法清洁级雄性SD大鼠 90只 ,随机分为正常对照组、假手术组、脑缺血再灌注组。用线栓法制备局灶性脑缺血再灌注模型。动物处死后测脑含水量 ,并用RT PCR方法测补体C3mRNA表达值。结果缺血侧补体C3mRNA表达增高 ,并与脑含水量之间呈正相关关系 (r =0 .4 6 6 ,P <0 .0 5 )。结论大鼠脑缺血再灌注中有补体系统的激活 。 ObjectivesTo investigate the activation and effects of complements in cerebral ischemia reperfusion.MethodsNinety healthy male SD rats were divided randomly into normal control group, sham operated group and ischemia reperfusion group. Cerebral ischemia reperfusion models were prepared by occlusion of the middle cerebral arteries using thread embolizing method. Rats were killed and their brain water contents (BWC) were measured. The expressions of C3 complement and apoJ mRNAs were estimated by RT PCR.ResultsThe expression level of C3 mRNA in rats of ischemia reperfusion group increased and a positive correlation was found between the expression level of C3 mRNA and BWC (r=0.466,P<0.05).ConclusionComplement system may be activated in cerebral ischemia reperfusion of rats, suggesting that this system could participate in the damage of rat brain tissue.
作者 罗建忠 朱文斌
机构地区 长沙市第一医院 中南大学湘雅医院神经内科
出处 《医学临床研究》 CAS 2003年第6期411-413,共3页 Journal of Clinical Research
关键词 脑缺血 再灌注损伤 补体激活 实验研究 疗效 cerbral ischemia reperfusion complement activation
分类号 R743.31 [医药卫生—神经病学与精神病学]
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参考文献12

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同被引文献23

  • 1韩英,刘楠,陈荣华,郑安,黄华品.白细胞介素8在大鼠脑缺血再灌注损伤中的变化[J].中国动脉硬化杂志,2005,13(2):155-157. 被引量：16
  • 2[8]Souza DG,Teixeira MM.The balance between the production of tumor necrosis factor-alpha and interleukin-10 determines tissue injury and lethality during intestinal ischemia and reperfusion.Mem Inst Oswaldo Cruz,2005,100(Suppl 1):59～66
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  • 7[15]Noto T,Furuichi Y,Ishiye M,et al.Tacrolimus (FK506) limits accumulation of granulocytes and platelets and protects against brain damage after transient focal cerebral ischemia in rat.Biol Pharm Bull,2007,30(2):313～317
  • 8[16]Mocco J,Choudhri T,Huang J,et al.HuEP5C7 as a humanized monoclonal anti-E/P-selectin neurovascular protective strategy in a blinded placebo-controlled trial of nonhuman primate stroke.Circ Res,2002,91(10):907～914
  • 9[17]Ruehl ML,Orozco JA.Stroker MB,et al.Protective effects of inhibiting both blood and vascular selectins after stroke and reperfusion.Neurol Res,2002,24(3):226～232
  • 10[18]Schafer MK,Schwaeble WJ,Post C,et al.Complement C1q is dramatically up-regulated in brain microglia in response to transient global cerebral ischemia.Immunol,2001,164(10):5446～52

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医学临床研究
2003年 第6期

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