摘要
目的 观察c erbB 2反义寡核苷酸 (ASODN)对胃癌细胞株SGC790 1细胞的影响。方法 采用脂质体c erbB 2反义 (ASODN)组及空白对照、错配 (SCODN)组转染SGC790 1细胞 ,采用Westernblot观察c erbB 2蛋白表达的变化 ,采用四氮唑盐 (MTT)比色法测定细胞增殖以及对顺铂细胞毒性的影响。结果 转染ASODN能特异性下调c erbB 2蛋白表达和抑制胃癌细胞增殖。此外 ,顺铂在空白组、ASODN组及SCODN组的抑制率分别为 ( 3 3 .3± 2 .0 ) %、( 4 9.9± 5 .9) %和 ( 3 4.8± 3 .0 ) %。与空白对照组相比 ,ASODN组的抑制率显著增加 (P <0 .0 1)。结论 c erbB
Objective To explore the effects of c erbB 2 antisense oligodeoxynucleotides (ASODN) in gastric cancer cell line SGC7901. Methods c erbB 2 ASODN and controlled oligonucleotides (blank and mismatched) were transfected by lipofectin, and then Western blot was taken to observe the changes of c erbB 2 protein expression.The cell proliferations and cytotoxity of cisplatin were assessed by MTT. Results c erbB 2 ASODN could specifically reduce the c erbB 2 protein expression and inhibit the cellular proliferation in human gastric cancer cells line ( P <0.01). Besides,the inhibition rates of cisplatin in the blank and mismatched groups were (33.3±2.0) %,(49.9±5.9)% and (34.8±3.0)%, respectively. Compared with the controls, the inhibition rate of antisense group increased significantly ( P <0.01). Conclusion The data suggest that the c erbB 2 ASODN can inhibit the proliferation of gastric cancer cell line and enhance the cytotoxicity to cisplatin.
出处
《上海医学》
CAS
CSCD
北大核心
2003年第6期312-314,共3页
Shanghai Medical Journal
