摘要
目的:研究体外诱导小鼠胚胎干细胞(embryonic stem cell,ESC)分化为结膜上皮细胞的可能性及条件,为构建组织工程化结膜提供新型的种子细胞奠定基础。方法:以人结膜上皮细胞作为诱导条件,采用分层共培养的方法诱导小鼠ESC,倒置相差显微镜观察共培养后形态的改变;免疫组织化学法检测诱导后的细胞角蛋白3/12(CK3/CK12)、角蛋白13(CK13)、MUC4和MUC5AC的表达。结果:采用人结膜上皮细胞作为诱导条件,ESC在诱导72h后可见部分细胞显示典型上皮样细胞形态,免疫组化检测CK13、MUC4表达阳性,CK3/CK12,MUC5AC表达阴性。结论:采用分层共培养的方法,用人结膜上皮细胞作诱导剂可以诱导小鼠ESC向结膜上皮样细胞分化。眼科学报2003;19:117-121。
Purpose: To investigate the probabilities of murine embryonic stem cells (ESC) differentiating into conjunctival epithelial cells and lay a basis for seeking a new source of seed cells provided in conjunctival tissue engineering.
Methods:In vitro, the ESC were induced by conjunctival epithelial cells in tran-swell . The morphological changes of the ESC were observed under phase contrast microscope , the specific markers of the induced cells were identified immunocytochemi-cally with Cytokeratin 3/12 (CK3/CK12) , Cytokeratin 13 (CK13), MUC4 and MUC5AC.
Results:The ESC might be induced by human conjunctival epithelial cells into the conjunctival epithehal-like cells after 72 hours of inducing.These cells had the typical epithelial cells morphology and were positive in cytokeratin 13、MUC4 staining, nega-tivein CK3/CK12、MUC5AC staining.
Conclusions The murine ESC may be induced into conjunctival epithelial-like cells by conjunctival epithelial cells of human in vitro. Eye Science 2003 ;19:117-121.
出处
《眼科学报》
2003年第2期117-121,共5页
Eye Science
基金
国家重点基础研究发展规划项目(973)(G1999054301)
国家自然科学基金(30171001
30000187
39770789
39700153
30200306)
广东省自然科学基金(990092
20010746)
