摘要
采用CTAB法从猕猴桃干叶片提取总DNA ,运用PCR技术扩增出rbcL和psbA两个叶绿体基因片段 ,分别用两种限制性内切酶对它们进行酶切分析。实验结果表明 :当CTAB提取液体积数为 75 0 μl,猕猴桃叶片质量为 10mg时 ,所得到的猕猴桃DNA的质量较为理想 ;DNA模板量为 2 5 0ng时 ,扩增到的特异性条带明亮、无拖尾 ;2个基因的酶切结果共得到 2 5个限制性位点 ,其中 2 4个具有多态性 。
The total DNA was extracted from dried leaf of Actindia by CTAB method Two chloroplast DNA fragments(rbcL gene and psbA gene were amplified by PCR and digested by two restriction endonucleaseas respectively The results showed that extracted DNA was much perfect when the volume of CTAB extract was 750μl with 10mg leaf of Actindia ;PCR-amplified bands were bright and without extra tail when the quantity of DNA template was 250ng;25 restriction siteswere found and 24 of them were polymorphic,which set up a solid foundation to explore phylogene in Actindia from cpDNA level
出处
《生物技术》
CAS
CSCD
2003年第3期10-11,共2页
Biotechnology
基金
国家自然科学基金资助项目 (39960 0 0 7)
江西省自然科学基金资助项目 (0 2 30 0 0 1 )
