摘要
替代寄主 (Coleoptera)被川硬皮肿腿蜂 (SclerodermasichuanensisXiao)雌成蜂寄生后第 10d ,虫体肌肉组织的结构、与骨骼的连接、脂肪组织以及消化道、马氏管、气管等组织系统均发生了明显的变化。同时寄主机体蛋白、氨基酸含量和蛋白水解酶活力等发生了显著的变化 :寄生后第 3～ 6d ,寄主体内蛋白质含量急剧下降 ,第 10d达最低值 340 8780mg·g- 1 ,比寄生当天降低了 17 0 0 % ;而游离氨基酸的含量则逐渐上升 ,第 10d达最大值 2 2 36 2 7mg·g- 1 ,比寄生当天增加了 4 2 4 5 % ,其中Ser、Gly、Ala、Val、Met、Phe、Ile、Leu、Lys、Trp等 10种游离氨基酸组分变化趋势与游离氨基酸总量的变化趋势一致 ;在所检测的 17种游离氨基酸中 ,各寄生时期含量最高者除寄生后第 1d为Tyr外 ,第 3、6、10d均为Ala ,且其含量变化明显 ;而对照各期含量最高者均为Tyr。寄生后第 1～ 3d ,蛋白酶活力迅速上升 ,第 3d达最大值 6 34 786 8μgTyr·g- 1 ·min- 1 ,然后逐渐下降 ,到第 10d接近寄生当天的水平。本文还就替代寄主的蛋白质与氨基酸代谢、川硬皮肿腿蜂的氨基酸营养生理等问题进行了讨论。
The structural, physiological and biochemical changes of the substitute host(Coleoptera)parasitized by Scleroderma sichuanensis were studied. The structure of the parasitized substitute host changed significantly on the 10th day. The content of protein and amino acids & the activity of proteinase changed remarkably after the substitute host parasitized by Scleroderma sichuanensis. The content of the protein decreased sharply on the 3rd to 6th day and got the minimum 340.878 0 mg·g -1 on the 10th day after parasitization. But the total content of the free amino acids increased step by step, so got the maximum 22 362 7 mg·g -1 on the 10th day, increased by 42 45% compared with the 1st day's. The content of Ser, Gly, Ala, Val, Met, Phe, Ile, Leu, Lys, Trp etc. changed as the total free amino acids content. The highest free amino acids content of parasitized alternative host pupae was Ala on the 3rd, 6th and 10th day, except the 1st day was Tyr with that of the control alternative host pupae being Tyr in all the period. The activity of proteinase elevated rapidly on the 1st to 3rd day after parasitization. It got the maximum 634 786 8?μg Tyr.g -1 ·min -1 on the 3rd day, then descended slowly to the level of the 1st day's. Then the metabolism of protein and amino acids of alternative host the nutritional physiology of S. sichuanensis larvae were discussed.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2003年第3期76-84,t001,t002,共11页
Scientia Silvae Sinicae
