摘要
目的 :探讨丙型肝炎时外周血T淋巴细胞亚群及可溶性白细胞介素 2受体 (sIL 2R)的变化。方法 :以输血后丙型肝炎 96例为研究对象 ,分别检测其外周血T淋巴细胞亚群及可溶性白细胞介素 2受体 ,对其中 18例患者进行α 干扰素(IFN α)抗病毒治疗 ,并于治疗前后观察此两项指标的变化 ,检测方法分别用流式细胞仪和双抗夹心ELISA法。结果 :丙型肝炎患者血清sIL 2R明显高于正常对照组 ,与血清ALT呈正相关 ;各型丙型肝炎时 ,CD4 + T细胞及CD4 + CD8+ 均下降 ,CD8+ T细胞升高 ;α 干扰素抗病毒治疗后 ,病毒复制指标阴转 ,同时可溶性白细胞介素 2受体水平下降 ,细胞免疫功能改善。结论 :免疫反应参与丙型肝炎发病 ,临床各型丙型肝炎均有免疫功能紊乱 ,观测T淋巴细胞亚群及可溶性白细胞介素 2受体的变化 ,是判断丙型肝炎转归和预后和干扰素疗效的有价值的指标。
Objective:To study the changes of serum T lymphocytes subgroup and soluble interleukin 2 receptors(sIL 2R) on patients with viral hepatitis C.Methods:T lymphocytes subgroup and soluble interleukin 2 receptor were detected in 96 cases suffering from different type of viral hepatitis C,and in 18/96 cases after the treatment of IFN α.The sIL 2R level and the T lymphocytes subgroup were detected by ELISA and flowcytometry(FCM),respectively.Results:The results show that the sIL 2R level is higher in the group of hepatitis C than the control group(P<0.05),and is correlated with the level of ALT.Comparing with the control group,the level of CD4 + T and CD4 +/CD8 + is lower and the level of CD8 + T is higher in all kinds of viral hepatitis C.After the therapy of IFN α,the level of reproduction of virus and the sIL 2R were descent,and the function of cellular immunity was improved.Conclusion:T lymphocytes subgroup and soluble interleukin 2 receptor have service abnormal in all types of viral hepatitis C.IFN α could improve the immunity.It is valuable to observe the prognosis of viral hepatitis C and effects of IFN α by using T lymphocytes subgroup and soluble interleukin 2 receptor.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2003年第6期431-433,共3页
Chinese Journal of Immunology
基金
太原市科委启明星项目资助
