摘要
目的 :建立PCR ELISA法检测PAF受体RNA。方法 :提取 2 0例银屑病病人和 2 0例正常对照组血液单个核细胞标本中的总RNA ,用双标记的PAF R(Biotion与Digoxin标记 )及 β actin(Biotin与Fluorescein标记 )引物进行PT PCR ,用ELISA检测PCR产物 ,用传统的琼脂糖凝胶电泳对照。结果 :ELISA法和琼脂糖凝胶电泳法对 β actin检测结果均为阳性 ;ELISA法检测PAF R ,2 0例病人标本均为阳性 ,2 0例对照组 16例阳性 ;琼脂糖凝胶电泳法检测PAF R ,2 0例病人标本 18例阳性 ,2 0例对照组 16例阳性。结论 :PCR ELISA引物双标记法检测PAF R操作简单 ,有较高的敏感性。
Objective:To study the methods of PCR ELISA in testing the RNA of platelet activating factor receptor.Methods:Extracting the total RNA of 20 normal people and 20 psoriasis patients,the PAF R and β actin RNA assayed by RT PCR,PAF R sense labelled by Biotin and antisense labelled by Digoxin,β actin sense labelled by Biotin and antisense labelled by fluorescein.The results of PCR were tested by ELISA and Agar gel electrophoresisa.Results:The positive number of PAF R RNA was 16 by Agar gel electrophoresis and 18 by PCR ELISA in 20 normal people;The positive number of PAF R RNA was 18 by Agar gel electrophoresisa and 20 by PCR ELISA in 20 Psoriasis patients;The positive number of β actin was 20 in two groups with two methods.Conclusion:The sense and antisense labelled by different substance in PCR ELISA has high sensitivity and the course was simple in testing the PAF R RNA.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2003年第6期415-417,共3页
Chinese Journal of Immunology
基金
贵州省科委基金资助项目 ( 993 0 5 1 C14 4)
