期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

TRAIL基因在白血病原代细胞中的表达

Expression of TRAIL gene in primary leukemic cells
在线阅读 下载PDF
导出
摘要 目的:检测白血病原代细胞和正常人外周血淋巴细胞中TRAIL基因的表达情况,探讨TRAIL凋亡途径在肿瘤免疫中的作用。方法:通过RT-PCR检测TRAIL基因的表达。结果:83%(10/12)淋巴系白血病和80%(16/20)髓系白血病患者TRAIL基因表达阳性,正常人外周血淋巴细胞TRAIL基因表达阴性,IL-2能诱导PBL的TRAIL基因表达。结论:大多数白血病原代细胞TRAIL基因表达阳性,可能在肿瘤的自发坏死和凋亡以及免疫逃逸中发挥作用。另外,活化的T细胞表达TRAIL基因,TRAIL可能参与CD95非依赖性激活诱导的细胞凋亡。 Objective:To determine the expression of TRAIL gene in primary leukemic cells and PBLs from normal individuals and study its possible role in tumor immunology.Methods:RT-PCR was used to determine TRAIL gene expression.Results:TRAILmRNA transcripts were detected in10/12lymphocytic leukemia and16/20myelocytic leukemia samples by RT-PCR.There was no detectable TRAIL mRNA in PBLs from normal individuals,and IL-2could induce its expression.Conclusion:Most primary leukemic cells expressed TRAIL,suggesting TRAIL might play a role in tumor cell evasion of immunosurveillance and spontaneous tumor cell death and necrosis.In addition,expression of TRAIL was found in activated T cells,suggesting that TRAIL might participate in CD95-independent apoptosis of lymphoid cells.
作者 王玲玲 张茂宏 徐从高
机构地区 山东大学护理学院内科学教研室 山东大学齐鲁医院血液科
出处 《山东大学学报（医学版）》 CAS 2003年第2期185-187,共3页 Journal of Shandong University:Health Sciences
关键词 基因 TRAIL 白血病细胞 肿瘤免疫 细胞凋亡 Gene,TRAIL Leukemic cells Tumor immunology Cell apoptosis
分类号 R730.3 [医药卫生—肿瘤] Q786 [生物学—分子生物学]
  • 相关文献

参考文献10

  • 1Wiley SR, Schooley K, Smolak PJ, et al. Identification and characterization of a new member of the TNF family that induces apoptosis [J]. Immunity, 1995, 3(6): 673.
  • 2Pan G, O'Rourke K, Chinnaiyan AM, et al. The receptor for the cytotoxic ligand TRAIL [J]. Science,1997, 276 (5309): 111.
  • 3Schneider P, Thome M, Burns K, et al. TRAIL receptors 1 (DR4) and 2 (DILS) signal FADD-dependent apoptosis and activate NF-kappaB [J]. Immunity, 1997,7 (6): 831.
  • 4Zhao S, Asgary Z, Wang Y, et al. Functional expression of TRAIL by lymphoid and myeloid tumor cells[J]. Br J Haematol, 1999, 106 (3): 827.
  • 5Snell V, Clodi K, Zhao S, et al. Activity of TNF-related apoptosis-inducing ligand (TRAIL) in haematological malignancies [J]. Br J Haematol, 1997, 99 (3): 618.
  • 6Koyama S, Koike N, Adachi S. Expression of TNF-related apoptosis-inducing ligand(TRAIL) and its receptors in gastric carcinoma and tumor-infiltrating lymphocytes: a possible mechanism of immune evasion of the tumor [J]. J Cancer Res Clin Oncol, 2002, 128(2):752.
  • 7Jeremias I, Herr I, Boehler T, et al. TRAllVApo-2-ligand-induced apoptosis in human T cells [J]. Eur J Immunol, 1998, 28 (1): 143.
  • 8Zhang M, Li X, Pang X, et al. Identification of a potential HIV-induced source of bystander-mediated apoptosis in T cells: upregulation of trail in primary human macrophages by HIV-1 tat [J]. J Biomed Sci,2001, 8(3): 290.
  • 9Miura Y, Misawa N, Maeda N, et al. Critical contribution of Tumor Necrosis Factor-related Apoptosis-induclig Ligand (TRAIL) to Apoptosis of Human CD4+ T cells in HIV-l-infeeted hu-PBL-NOD-SCID Mice [J]. J Exp Med, 2001, 193(5): 651.
  • 10Gibellini D, Re M C, Ponti C, et al. HIV-1 Tat protect CD4+ Jurkat T lymphoblastoid cells from apoptosis mediated by TNF-related apoptosis-inducing ligand[J]. Cell Immuno, 2001, 207(2): 89.
山东大学学报（医学版）
2003年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部