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竞争性核酸定量检测技术研究进展 被引量:1

A Studing make Progress on Quantitative Detection Technical of Competitive Nucleinic Acid
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摘要 在医学基础研究和临床应用的诸多领域中 ,由单纯PCR技术提供的定性结果存在着较多的局限性 ,对核酸进行基因及其表达等的定量检测是分子生物学发展的一大方向。因此 ,在对目前常用的定量方法进行简述的基础上 ,对其中应用最为广泛的竞争性核酸定量检测技术的原理、方法。
作者 叶巍 方筠
出处 《中国国境卫生检疫杂志》 CAS 2003年第1期51-52,共2页 Chinese Journal of Frontier Health and Quarantine
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  • 1邓展云,王伯辉,刘海斌,朱秋珍,李鸣,王维赞,谭裕模.广西甘蔗宿根矮化病的发生及病原检测[J].中国糖料,2004,26(3):35-38. 被引量:54
  • 2Hoy J, Grisham M P, Damann K E. Sread and Increase of ratoon stunting disease of sugarcane and comparison of disease detection method[J]. Plant disease, 1999, 83:1 170-1 175.
  • 3Gillaspie A G, Davis R E, Worley J E. Nature of the ratoon stunting disease agent[J]. Proceeding of the international society of Sugar Cane Technologists, 1974, 15: 218-224.
  • 4Grisham M P. Effect on ratoon stunting disease on yield of sugarcane grown in multiple three-year planting[J]. Phytopathology, 1991, 81: 337-340.
  • 5黄应昆.甘蔗主要病虫害原色图谱[M].昆明:云南科技出版社,2002:71-72.
  • 6Barry J, Croft A. Method for rating sugarcane cultivars for resistance to ratoon stunting disease based on an enzyme-linked immunoassay[J]. Australian Plant Pathology, 2002, 31(1): 63-66.
  • 7Uberla K, Platzer C, Diamantstein T, et al. Generation of competitor DNA fragments for quantitative PCR[J]. PCR Methods Appl, 1991, 1(2): 136-139.
  • 8Davis M J, Gillaspie A G, Harris R W, et al. Ratoon stunting disease of sugarcane: Isolation of the causal bacterium [J]. Science, 1980, 210: 1 365-1 367.
  • 9Pan Y B, Grisham M P, Burner D M, etal. A Polymerse Chain Reaction Rotocol for the Detection Clavibacter xyli subsp, xyli, the Causal Bacterium of Sugarcane Ratoon Stunting Disease[J]. Plant Disease, 1998, 82(3): 285-290.
  • 10Claudia B, Monteiro-Vitorello, Luis E A, et ol. The Genome Sequence of the Gram-Positive Sugarcane Pathogen Leifsonia xyli subsp, xyli[J]. Molecular plant-microbe interactions, 2004, 17(8): 827-836.

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  • 1邓展云,王伯辉,刘海斌,朱秋珍,李鸣,王维赞,谭裕模.广西甘蔗宿根矮化病的发生及病原检测[J].中国糖料,2004,26(3):35-38. 被引量:54
  • 2Hoy J, Grisham M P, Damann K E. Sread and Increase of ratoon stunting disease of sugarcane and comparison of disease detection method[J]. Plant disease, 1999, 83:1 170-1 175.
  • 3Gillaspie A G, Davis R E, Worley J E. Nature of the ratoon stunting disease agent[J]. Proceeding of the international society of Sugar Cane Technologists, 1974, 15: 218-224.
  • 4Grisham M P. Effect on ratoon stunting disease on yield of sugarcane grown in multiple three-year planting[J]. Phytopathology, 1991, 81: 337-340.
  • 5黄应昆.甘蔗主要病虫害原色图谱[M].昆明:云南科技出版社,2002:71-72.
  • 6Barry J, Croft A. Method for rating sugarcane cultivars for resistance to ratoon stunting disease based on an enzyme-linked immunoassay[J]. Australian Plant Pathology, 2002, 31(1): 63-66.
  • 7Uberla K, Platzer C, Diamantstein T, et al. Generation of competitor DNA fragments for quantitative PCR[J]. PCR Methods Appl, 1991, 1(2): 136-139.
  • 8Davis M J, Gillaspie A G, Harris R W, et al. Ratoon stunting disease of sugarcane: Isolation of the causal bacterium [J]. Science, 1980, 210: 1 365-1 367.
  • 9Pan Y B, Grisham M P, Burner D M, etal. A Polymerse Chain Reaction Rotocol for the Detection Clavibacter xyli subsp, xyli, the Causal Bacterium of Sugarcane Ratoon Stunting Disease[J]. Plant Disease, 1998, 82(3): 285-290.
  • 10Claudia B, Monteiro-Vitorello, Luis E A, et ol. The Genome Sequence of the Gram-Positive Sugarcane Pathogen Leifsonia xyli subsp, xyli[J]. Molecular plant-microbe interactions, 2004, 17(8): 827-836.

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