摘要
目的观察实验性脑卒中恢复期的神经行为学得分、患侧病灶周围以及健侧相应区域的神经胶质细胞的变化情况,为研究脑出血后遗症期工作打基础。方法用高血压大鼠制作脑出血模型,根据Bederson评定方法记录脑出血后1,3,6个月的神经行为学改变得分;同时在640倍镜下,在患侧脑病灶周围选5个视野对病灶周围的神经胶质细胞进行计数,然后算每个视野的均数和标准差;在健侧脑的同样部位也进行同样的计数和统计。结果(1)3个月内,正常血压脑出血组神经行为学得分从7.2±0.7下降到6.3±1.5(t=3.0689,P<0.01),6个月时下降到5.8±1.8(t=4.0982,P<0.01);高血压脑出血组神经行为学得分3个月内从8.5±0.7下降到7.3±0.9(t=3.5968,P<0.01),6个月时下降到6.1±0.5(t=8.0134,P<0.01)。(2)所有脑出血组术后6个月出现脑萎缩和脑皮质变薄。(3)所有脑出血组1,3,6个月时病灶周围及健侧相应区域的胶质细胞数量均较术前大量增加(P<0.01),且以3个月时为高峰。(4)神经行为学改变与患侧、健侧胶质细胞数变化之间存在明显相关性联系r=0.9879(P<0.01)和r=0.734(P<0.01)。结论(1)脑出血后确实存在着自然恢复的现象。但同时出现的脑萎缩,脑皮质变薄的现象提示似乎神经行为学的改善与脑皮质的功能关系并不大。(2)6个月内病灶周围的胶质细胞的增?
Aim To observe the changes of the neuroet hological scores and the number of neuroglial cell aro und the focus among the rats with ex-perimental cerebral hemorrhage dur ing the convalescent period so as to l ay a base for study of sequelae of cerebra l hemorrhage.Methods 270Wistar rats were randomly divided into norm al blood pressure group(group A,n=60)that was subdivided into group of normal control(group A0)and group of cerebral hemorrhage with normal blood pressure(group A1,collaginase and heparin were injected into the ca udate nucleus to produce hemorrhage),and group of renal hypertension(group B,n=210,the bilateral renal ar-teries were squeezed to establish th e model of renal hypertension),subdi-vided into control group(group B0,including groups B0-1,B0-3,and B0-4),sham operation group(group B1,normal saline was injected into the caudate nucleus),and group of cerebral hemorrhage(collaginase and hep-arin were injected into the caudate n ucleus to produce hemorrhage)groups B2,B3,and B4).The rats were killed one month after the hemor-rhage-producing operation(groups A0,A1,B1,B0-2,and B2),3months after(groups B0-3and B30,and 6months afte r(groups B0-4and B4)and their brains taken out to be fixed and stained by hematoxylin and eosin.Sl ices were made at the level of the foci.Fiv e fields of view were selected around a focus to undergo microscopy to count the number of neuroglial cells.The number of neuroglial cells in the corresponding sites of the contralateral side was counted too.The neuroethologic al scores 1,3,and 6months after the operation were marked by Bederson's method.Results(1)In the cerebral hemorrhage group with normal blood p ressure(group A1)neuroethological score descended from the baseline le vel of 7.23±0.71to 6.29±1.523months after injection(P<0.01)and 5.81±1.766months after(P<0.01).In the cerebral hemorrhage group wi th renal hypertension(group B )the neuroethological score descended from the baseline leve3l of 8.47±0.71to 7.31±0.93months after operation(P<0.01)and 6.11±0.546months after(P<0.01).(2)In all groups of cerebral hemorrhage,brain atrophy and thinning of cortex were s een 6months after the operation.In a ll groups of cerebral hemorrhage the number of neuroglial cells 1,3,and 6months after operation were greatly increased in comparison of that pre-operationally with a summit 3mo nths after operation(P<0.01).(4)There was an obvious correlation bet ween the changes of the neuroetholog ical score and hyperplasic response of neuroglial cells(r =0.9879,P<0.01;r =0.734,P<0.01).Conclusion(1)Spontaneous recovery exists after cerebral hemorrhage.But it seems th at there is not a direct relationship be-tween the improvement of neuroethol ogical scores and the function of bra incortex.(2)There are continuously hyperplasic responses of neuroglial cells in the brain during 6months after the operation.(3)Melioration of neu-roethology shows an obvious correla tion with hyperplasic response of neu-roglial cells.
出处
《中国临床康复》
CSCD
2003年第13期1888-1889,共2页
Chinese Journal of Clinical Rehabilitation
