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湖羊、同羊12个同工酶座位的检测 被引量:2

Genetic estimation of 12 isozymes in Hu sheep and Tong sheep
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摘要 Gene frequencies of Hu sheep and Tong sheep were obtained with“Random sampling in typical colonies of a central area”. Of the 12 loci tested in Hu sheep, 11 loci were polymorphic. Reliability of the estimated frequencies of 27 alleles reached 0.95 except for PoF, TfA,Tff,Hb-βA and CAF which had reliabilities of 0.5222,0.7478,0.5222,0.6212 and 0.899, respectively. Of the 12 loci tested in Tong sheep, 11 loci were polymorphic. Reliability of the estimated frequency of 25 alleles reached 0.95 except for TfA, TfE and CAF which had reliabilities of 0.931,0.6922 and 0.7924, respectively. The average heterozygosity () and average homozygosity (J) was computed and the J of the two sheep colonies was 0.6619 and 0.6448, respectively. Consistent with our conclusions based on genetic data, previous research divided the native sheep populations of East and South Central Asia into three group: the“Mongolian group”,“South-Asian group” and“European group”. Consequently, the degree of genetic similarity between populations and known groups would seem to provide a reliable means of determining the genetic relationships between populations and may reflect the true genetic origin of Hu sheep and Tong sheep in China [Acta Zoologica Sinica 49(1):134-138,2003]. Gene frequencies of Hu sheep and Tong sheep were obtained with“Random sampling in typical colonies of a central area”. Of the 12 loci tested in Hu sheep, 11 loci were polymorphic. Reliability of the estimated frequencies of 27 alleles reached 0.95 except for PoF, TfA,Tff,Hb-βA and CAF which had reliabilities of 0.5222,0.7478,0.5222,0.6212 and 0.899, respectively. Of the 12 loci tested in Tong sheep, 11 loci were polymorphic. Reliability of the estimated frequency of 25 alleles reached 0.95 except for TfA, TfE and CAF which had reliabilities of 0.931,0.6922 and 0.7924, respectively. The average heterozygosity () and average homozygosity (J) was computed and the J of the two sheep colonies was 0.6619 and 0.6448, respectively. Consistent with our conclusions based on genetic data, previous research divided the native sheep populations of East and South Central Asia into three group: the“Mongolian group”,“South-Asian group” and“European group”. Consequently, the degree of genetic similarity between populations and known groups would seem to provide a reliable means of determining the genetic relationships between populations and may reflect the true genetic origin of Hu sheep and Tong sheep in China [Acta Zoologica Sinica 49(1):134-138,2003].
出处 《动物学报》 SCIE CAS CSCD 北大核心 2003年第1期134-138,共5页 ACTA ZOOLOGICA SINICA
基金 国家自然科学基金国际研究项目 (No .30 2 130 0 9)资助~~
关键词 湖羊 同羊 同工酶 座位 检测 遗传检测 血统归属 Hu sheep, Tong sheep, genetic estimation, Breed identification
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参考文献11

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  • 2Chang, H., S- M. Geng, B. Wu, X. Y. Li, Y. C. Chen 1989 Studies of efficiency of sampling estimation for gene frequencies in Chinese cattle breeds. Acta Universitatis Septentrionali Occidentali Agriculture 17 (3): 30--37.
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同被引文献19

  • 1常国斌,常洪,刘向萍,杨章平,陈国宏,赵文明,薛燕,黄峰.中国野生鹌鹑群体遗传共适应特性研究[J].中国科学(C辑),2005,35(5):452-461. 被引量:10
  • 2Kenji Tsunoda.Genetic Characteristics of Bangladeshi Sheep as Based on Biochemical variations[J].Zootech.Sci.,1989 61(1):54-66.
  • 3S.X.Lu,H.Chang et al.Genetic differentiation among sheep population from near-sea mainland in east Asia[J].Asia-Aut.J.Anima.Sci,2004,(17):1360-1365.
  • 4Irié Zoro Bi.Genetic control of isozymes in the primary gene pool Phaseolus lunatus L[J].Biotechnol.Agron.Soc.Environ,1999,3(1),10-27.
  • 5R.Mark Hardisty and William Caire.Electrophoretic Band Patterns of Phosphoglucoisomerase and Malate Dehydrogenase in the Prickly Pear Cactus Opuntia cymochila Engelm.and O.phaeacantha Engelm.(Cactaceae) from Southwestern Oklahoma[J].Proc.Okla.Acad.Sci,1989,(69):5-9.
  • 6胡国能,万贤国.同工酶技术及其应用[M].长沙:湖南科学出版社,1985 123-125.
  • 7刘玲玲,李悦民.暗纹东方同工酶生化表现型的研究江苏省、浙江省生物化学学术交流会论文集[C].1999:49-52.
  • 8GENG RQ, CHANG H, YANG ZP, SUN W, WANG LP,LU SX, Tsunoda K. Study on origin and phylogeny stares of Hu Sheep. Asian-Aust J Anim Sci, 2003, 16( 5): 743--747.
  • 9Tsunoda Kenji, Doge K, Yamamoto Y. Biochemical polymorphisms of Nepalese native sheep breeds. Anim Sci Technol (Jpn.), 1993, 64:1051 -- 1059.
  • 10常洪,耿社民,武彬,李写一,陈幼春.中国黄牛品种基因频率抽样估计效率的研究[J].西北农林科技大学学报(自然科学版),1989,23(3):30-37. 被引量:31

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