摘要
为探讨维生素E (VE)对中华鳖 (Pelodiscussinensis)幼鳖的生长、肝脏VE和血清皮质醇的影响 ,通过特定生长率、高压液相色谱法和放免法 ,我们测定了中华鳖幼鳖的生长、肝脏VE和血清皮质醇含量。发现VE添加量为 10 0 0和 5 0 0 0mg/kg的两组 ,能明显降低中华鳖幼鳖的生长。维生素E添加量为 5 0 0、 10 0 0和5 0 0 0mg/kg的三组 ,肝脏维生素E含量明显高于对照组 ,VE添加量在 0～ 10 0 0mg/kg的范围时 ,肝脏VE的含量随着饲料中VE含量的增加呈指数式增加 ,并且在VE添加量为 5 0 0 0kg/kg的一组基本达到饱和。维生素E添加量为 0和 5 0mg/kg的 2组 ,其血清皮质醇的平均值明显高于维生素E添加量为 2 5 0、 5 0 0、 10 0 0和 5 0 0 0mg/kg的 4组的平均值。上述结果表明 :高剂量的VE降低了中华鳖幼鳖的生长和血清皮质醇的含量 ;在一定剂量范围内 ,肝脏VE随着饲料中VE含量的增加而升高 [动物学报 49(1) :40～ 44 ,2 0 0 3]。
To investigate the effects of vitamin E on growth, liver vitamin E and the serum cortisol level of juvenile soft-shelled turtles, we estimated the growth in juvenile soft-shelled turtles by specific growth rate, analyzed liver vitamin E with a high pressure liquid chromatogram (HPLC) and determined serum cortisol content using the radiate immune method. Growth in juvenile soft-shelled turtles was depressed in two groups that received vitamin E supplementation at 1 000 and 5 000 mg/kg of diet. Levels of liver vitamin E in turtles that received vitamin E supplementation at 500, 1 000 and 5 000 mg/kg of diet were significantly higher than in the control group. The level of liver vitamin E increased exponentially with increasing dietary vitamin E within the range 0-1 000 mg/kg of diet, reaching saturation when dietary vitamin E reached 5 000 mg/kg of diet. The mean serum cortisol content in two groups that received vitamin E supplementation at 0 and 50 mg/kg of diet was significantly higher than that in four groups supplemented at 250, 500, 1 000 and 5 000 mg/kg of diet. These results suggest that high doses of vitamin E reduce growth rates and serum cortisol levels in juvenile soft-shelled turtles, and that liver vitamin E increases with increasing dietary vitamin E within certain limits[Acta Zoologica Sinica 49(1):40-44,2003].
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2003年第1期40-44,共5页
ACTA ZOOLOGICA SINICA
基金
中国科学院水生生物研究所淡水生态和生物技术国家重点实验室开放课题资助.~~
关键词
维生素E
中华鳖
幼鳖生长
肝脏VE
血清皮质醇
Soft-shelled turtle (Pelodiscus sinensis), Vitamin E, Growth, Liver, Cortisol
