摘要
从北京地区公园湖岸土壤中分离到一株橙红色杆状耐辐射菌 ,细胞壁革兰氏染色为阴性 ,电镜显示菌体大小为 0 6μm～ 1 6μm ,略大于日本学者报道的Deinobactergrandis菌 ,过氧化氢酶的含量和分子量不同于D .radioduransR1菌 ,分离菌的 (G +C)mol%含量为 70 7% ,1 6SrDNA序列分析表明 ,分离到的杆状耐辐射菌 (RR5 33 2 ) 1 6SrRNA基因序列与Deinobactergrandis菌高度同源 ,提示RR5 33 2归于Deinobacter菌属 ,并可能是该菌属中的一个新种。
A novel radiation-resistant bacterium was isolated from soil of lake bank in Beijing. The bacterium produced orange-pigmented colonies and formed rod-shaped cells that stained gram negative alike the Deinobacter grandis previously described by Japan's scientist. It was found with electron microscopy that the isolate is of 0.6μm～1.6μm size and has a 30～40nm thickness of cell wall, being slightly larger and thicker than the Deinobacter grandis. There was a difference in the concentration and molecule weight of catalase between the isolate and the Deincoccus radiodurans R1. The deoxyribonucleic acid guanine plus cytosine(G+C) base ratio was 70.7mol%. 16S RNA gene sequencing also showed that this rod-shaped bacterium possessed a high homology with the Deinobacter grandis,suggesting that it might be classified into the genus Deinobacter and constitute a new species in this genus.
出处
《微生物学报》
CAS
CSCD
北大核心
2003年第3期301-307,共7页
Acta Microbiologica Sinica
基金
国家自然科学基金资助项目 ( 30 0 70 2 0 1 )~~
关键词
短杆状耐辐射菌
鉴定
细菌分离
辐射
Isolation of bacterium, Radiation-resistant bacterium, Radiation
