摘要
目的 研究小鼠皮质神经元类缺血再灌注损伤后Bax的表达情况 ,探讨Bax的表达与神经元凋亡的关系。方法 利用流式细胞仪对培养的皮质神经元类缺血再灌注损伤后Bax的表达情况进行研究 ,利用原位末端标记法(TUNEL)对类缺血再灌注后不同时间点的神经元进行检测。结果 缺血再灌注 2h为Bax的表达高峰 ,而后随着再灌注时间的延长 ,Bax的表达下降 ,TUNEL表达高峰在再灌注 6h。结论 类缺血再灌注处理培养的皮质神经元Bax的表达高峰早于TUNEL法高峰。
Objective To explore the expression of Bax in mouse cerebral cortical neurons under ischemia-like condition and to investigate the relation between the expression of Bax and apoptosis.Methods The expression of Bax in cultured cortical neurons under ischemia-like condition was detected by flow cytometer.Apoptosis in cortical neurons under ischemia-like condition was detected by TUNEL method.Results The peak of the expression of Bax appeared at 2 hours of reperfusion.Then the positive neurons of Bax decreased with increasing the time of reperfusion.The peak of the positive neurons of TUNEL appeared at 6 hours of reperfusion.Conclusion The expression of Bax appears earlier than TUNEL in cortical neurons under ischemia-like condition.
出处
《中华老年心脑血管病杂志》
CAS
2003年第3期198-200,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
关键词
脑缺血
再灌注
神经元
细胞凋亡
brain ischemia
reperfusion
neurons
apoptosis
