摘要
近年来 ,动物克隆技术发展迅速 ,不断有新的克隆动物面世。面对克隆效率、克隆动物存活率低的客观事实 ,人们对克隆有关机制做了很多探讨。在移植核的再程序化过程中 ,某些胞浆因子如核质原等在解除已分化细胞核染色质的抑制中发挥了关键的作用 ,同时移植核发生印记基因及非印记基因的去甲基化和重新甲基化的现象 ,这种染色质抑制作用的解除及基因的甲基化现象与移植核的去分化有着密切的联系 ,但其具体机制还不清楚。核移植技术中 ,供核与受体胞质的协调和核再程序化的关系得到大量研究 ,目前普遍认为为了使核再程序化进行得更完全 ,MⅡ期卵母细胞质是适宜的受体 ,而处于G0期或G1期的体细胞核是合适的核供体。
Despite the recent rapid development in animal cloning technology and increased production of cloned animals, the cloning and survival rate of cloned animals has remained very low. In face of this fact, researchers have invested considerable effort in investigating alternative cloning mechanisms. In the procedure of involving the reprogramming of a transplanted nucleus, some cytoplasmic factors, such as nucleoplasmin, play key roles in the erasure of the repressive chromatin accompanied by DNA demethylation and re-methylation of imprinting and non-imprinting genes. Researchers have known that these phenomena correlate with the reprogramming of transferred nucleus, but the mechanism is not yet clear. At the same time, the compatibility of the transferred nucleus and receptive cytoplasm is very important to normal reprogramming. At present, the cytoplasm of an MⅡ phase oocytes is used as a suitable recipient and the somatic nucleus in G 0/G 1 phase is used as a suitable donor nucleus.
出处
《动物学杂志》
CAS
CSCD
北大核心
2003年第3期100-104,共5页
Chinese Journal of Zoology
