摘要
目的 :探讨粉防己碱 (Tet)对离体新西兰白兔阴茎海绵体的松弛作用。 方法 :采用离体新西兰白兔阴茎海绵体肌条张力记录法 ,观察Tet对氯化钾 (KCl)和去氧肾上腺素 (PE)诱导收缩的阴茎海绵体肌条的松弛作用 ;L 硝基精氨酸 (L NNA)和亚甲蓝处理后 ,Tet对PE诱导收缩的阴茎海绵体肌条松弛的作用。 结果 :10 μmol/L及3 0 μmol/L的Tet使KCl诱导的最大收缩反应分别降低为 ( 73 .0± 3 .8) %和 ( 41.5± 3 .4) % ,降低程度与Tet的浓度呈正比 (P <0 .0 1)。Tet对 10 μmol/LPE诱导的肌条收缩具有浓度依赖性松弛作用 ,1、10、3 0、10 0 μmol/L的Tet对PE诱导的阴茎海绵体肌条收缩的松弛效应分别为 ( 6.0± 1.4) %、( 2 1.3± 2 .2 ) %、( 47.4± 3 .3 ) %和 ( 68.1±3 .6) % (P <0 .0 1) ;而L NNA及亚甲蓝对Tet的松弛作用没有影响 (P >0 .0 5 )。 结论 :Tet能浓度依耐性地松弛离体新西兰白兔阴茎海绵体 ,其作用机制可能是通过阻滞钙通道 ,而与一氧化氮 环鸟苷酸 (NO cGMP)
Objectives: To study the relaxation effects of tetrandrine on the corpus cavernosum tissue of rabbit in vitro. Methods: ①The fluctuation of the dose-response relaxation curves for the contraction of KCl induced by tetrandrine was observed with isolated rabbit corpus cavernosum tissue.②Isolated strips of rabbit corpus cavernosum tissue were precontracted with 10 μmol/L phenylephrine(PE). Relaxation in response to cumulative doses of tetrandrine was determined in the absence and presence of nitric oxide synthase inhibitor (L-NNA) and soluble guanylate cyclase inhibitor (methylthioninium). Results: ①The dose-response curves of KCl were shifted to the right nonparallelly, and the maximal responses were depressed to ( 73.0± 3.8)% and ( 41.5± 3.4)%, respectively, in the presence of tetrandrine(10 μmol/L,30 μmol/L). ②On rabbit cavernosal muscle stripes precontracted with PE(10 μmol/L), increasing concentrations of tetrandrine (1 μmol/L, 10 μmol/L, 30 μmol/L and 100 μmol/L) showed dose dependent relaxation [( 6.0± 1.4)%, ( 21.3± 2.2)%, ( 47.4± 3.3)%, and ( 68.1± 3.6)%, P< 0.01]. However, in the meantime, it was found that these relaxation effects were not affected by the presence of L-NNA and methylthioninium (P> 0.05). Conclusions: Tetrandrine was effective in relaxing rabbit corpus cavernosum tissue in vitro in a dose-dependent style. The mechanism might be related with its blocking effect on calcium channel, but not the NO-cGMP passage. Natl J Androl,2003,9(3):234-236
出处
《中华男科学杂志》
CAS
CSCD
2003年第3期234-236,共3页
National Journal of Andrology
关键词
粉防己碱
阴茎海绵体
离体
钙通道
Tetrandrine
Corpus cavernosum, in vitro
Calcium channel
